Clinical laboratory evaluation of Ames' Seralyzer Reflectance Photometer for total bilirubin and cholesterol.

We evaluated the dry-reagent technology for determining cholesterol and adult total bilirubin in serum with the Ames Seralyzer Reflectance Photometer. This instrument is designed to measure the intensity of light of a selected wavelength that is reflected from reagent-impregnated test strips saturated with sample. Regression analysis of the data for total bilirubin in adults (y) compared with corresponding values obtained with the Technicon SMAC (x) yielded the regression line: y = 1.07x + 2.5 (n = 128, r = 0.99). Day-to-day precision (CV) was 3.8% at 8.1 mg of bilirubin per liter, 5.34% at 48.3 mg/L. Regression analysis of the cholesterol data (y) vs cholesterol values obtained with an enzymatic cholesterol method (x) yielded the regression line: y = 0.91x + 191 mg/L (n = 100, r = 0.98). Day-to-day CVs for cholesterol were 3.6% at 1.62 g/L, 3.11% at 2.62 g/L.