Fischer J C, Ruitenbeek W, Stadhouders A M, Trijbels J M, Sengers R C, Janssen A J, Veerkamp J H
Clin Chim Acta. 1985 Jan 15;145(1):89-99. doi: 10.1016/0009-8981(85)90022-1.
A method is presented which allows the investigation of almost the complete mitochondrial content of small human skeletal muscle biopsy specimens. Thorough mechanical disruption with a chopper apparatus results in the release of about 50% of the mitochondrial content. Subsequent treatment of the 600 x g sediment with trypsin releases another 30% of the total mitochondrial population. The biochemical characteristics of the two mitochondrial fractions obtained in these two successive steps have been compared. No obvious differences could be established. The procedure is well suited for biochemical investigation of muscle biopsy specimens from patients suspected of suffering from a mitochondrial myopathy.
本文介绍了一种方法，该方法能够对取自人体小块骨骼肌活检标本中几乎全部的线粒体成分进行研究。使用切碎器装置进行充分的机械破碎可使约50%的线粒体成分释放出来。随后用胰蛋白酶处理600×g离心沉淀物，可再释放出30%的线粒体总数。已对在这两个连续步骤中获得的两个线粒体组分的生化特性进行了比较。未发现明显差异。该方法非常适合对疑似患有线粒体肌病患者的肌肉活检标本进行生化研究。
