Sánchez-González María Carmen, Gallardo-Real Inmaculada, Gutiérrez-Sánchez Estanislao, De-Hita-Cantalejo Concepción, Capote-Puente Raúl, Sánchez-González José-María
Department of Physics of Condensed Matter, Optics Area, University of Seville, Reina Mercedes S/N, 41012 Seville, Spain.
Department of Physics of Condensed Matter, Optics Area, University of Seville, Reina Mercedes S/N, 41012 Seville, Spain.
Cont Lens Anterior Eye. 2025 Jun;48(3):102368. doi: 10.1016/j.clae.2025.102368. Epub 2025 Jan 8.
To characterize the ocular surface microbiota in regular contact lens wearers with dry eyes and assess the effectiveness of reducing bacterial load using a liposomal ozonated oil solution.
This prospective, longitudinal, controlled study randomized subjects into two groups. Group A (45 subjects) received hydroxypropylmethylcellulose (HPMC, Artific®), while Group B (41 subjects) received ozonated sunflower seed oil with soybean phospholipids (OSSO, Ozonest®). Microbial communities were analyzed via DNA metabarcoding of the 16S rRNA gene, and statistical analyses (alpha and beta diversity) were performed in R.
Both groups predominantly harbored Staphylococcus caprae, Streptococcus oralis, and Corynebacterium spp., with OSSO and HPMC users showing distinct bacterial profiles. Alpha diversity showed no significant differences, but beta diversity revealed differences in bacterial composition between the groups.
The results seem to indicate that the use of ozonized oil reduces the bacterial load compared to the solution used as a control.
对患有干眼症的长期佩戴隐形眼镜者的眼表微生物群进行特征分析，并评估使用脂质体臭氧油溶液降低细菌载量的有效性。
这项前瞻性、纵向、对照研究将受试者随机分为两组。A组（45名受试者）使用羟丙基甲基纤维素（HPMC，Artific®），而B组（41名受试者）使用含有大豆磷脂的臭氧葵花籽油（OSSO，Ozonest®）。通过对16S rRNA基因进行DNA宏条形码分析微生物群落，并在R软件中进行统计分析（α和β多样性）。
两组主要都含有山羊葡萄球菌、口腔链球菌和棒状杆菌属，使用OSSO和HPMC的用户表现出不同的细菌谱。α多样性无显著差异，但β多样性显示两组之间细菌组成存在差异。
结果似乎表明，与用作对照的溶液相比，使用臭氧油可降低细菌载量。
