Nandi Indrani, Srivastava Pradeep, Chandra Pranjal
School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
ACS Appl Bio Mater. 2025 Jan 20;8(1):741-753. doi: 10.1021/acsabm.4c01569. Epub 2025 Jan 9.
Bone is a dynamic tissue that serves several purposes in the human body, including storing calcium, forming blood cells, and protecting and supporting the body's organs. Alkaline phosphatase (ALP) is secreted into the circulation by osteoblasts, the cells responsible for making bone. It attaches to the surface of osteoblast cells or matrix vesicles. Accordingly, ALP is present in serum and is a popular biomarker for the evaluation of bone disease and other disorders in clinical evaluations. In this study, a composite probe made of bioactive glass (BG) and multiwalled carbon nanotubes (MWCNT) was used to detect ALP through electrochemical impedance spectroscopy (EIS) without the need for labels. By combination of physical methods with electrochemical analysis, the biosensor probe was characterized. The analytical performance of the biosensor was evaluated using EIS, and the results revealed that it has a limit of detection (LOD) of 2.29 ± 0.35 U/L and a linear dynamic range (LDR) of 25-600 U/L; both of which are within the acceptable range for ALP detection in clinics. When tested against serum interfering chemicals, the biosensor probe that was designed shows a strong selectivity for ALP ( < 0.06). In addition, human serum and fetal bovine serum were used to test the system's ability to detect ALP in biological samples.
骨骼是一种动态组织，在人体中发挥多种作用，包括储存钙、形成血细胞以及保护和支撑身体器官。碱性磷酸酶（ALP）由成骨细胞分泌进入血液循环，成骨细胞是负责生成骨骼的细胞。它附着在成骨细胞表面或基质小泡上。因此，ALP存在于血清中，是临床评估中用于评估骨病和其他疾病的常用生物标志物。在本研究中，一种由生物活性玻璃（BG）和多壁碳纳米管（MWCNT）制成的复合探针被用于通过电化学阻抗谱（EIS）检测ALP，无需标记。通过物理方法与电化学分析相结合，对生物传感器探针进行了表征。使用EIS评估了生物传感器的分析性能，结果表明其检测限（LOD）为2.29±0.35 U/L，线性动态范围（LDR）为25 - 600 U/L；这两者都在临床检测ALP的可接受范围内。当针对血清干扰化学物质进行测试时，所设计的生物传感器探针显示出对ALP具有很强的选择性（<0.06）。此外，使用人血清和胎牛血清测试了该系统检测生物样品中ALP的能力。
