Kalligosfyri Panagiota M, Miglione Antonella, Esposito Alessia, Alhardan Raghad, Iula Gabriella, Atay Iclal, Darwish Ibrahim A, Kurbanoglu Sevinc, Cinti Stefano
Department of Pharmacy, University of Naples Federico II, 80131, Naples, Italy.
Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06560, Ankara, Türkiye.
ChemistryOpen. 2025 Jun;14(6):e202500113. doi: 10.1002/open.202500113. Epub 2025 Apr 13.
Alkaline phosphatase (ALP) is an enzyme present in the human body responsible for the dephosphorylation of phosphorylated chemical species. It is primarily expressed in organs such as bones, liver, intestine, and placenta during pregnancy, playing a crucial role in cellular processes like gene expression, transport, and metabolism. Physiological ALP levels vary with age and sex, with normal serum ranges for healthy adults between 40 and 190 U/L. Alterations in ALP levels can be indicative of several pathologies, including cancer diagnosis and metastasis, as well as bone growth dysfunctions and hypophosphatasia. Conventional methods for ALP detection often require complex assay principles, extensive sample pretreatment, and trained personnel. Herein, the development of a portable, flexible electrochemical sensor fabricated through screen-printing to monitor ALP levels in biological samples is introduced. The flexible electrochemical sensor, characterized by high efficiency, sustainability, low cost, and ease of disposal, achieves detection limit as low as 0.03 and 0.08 U/L, respectively, in buffer solution and human serum samples, and a satisfactory repeatability lower than 10%. This simple sensor configuration approach enables real-time disease monitoring and improves access to point-of-care diagnostics, paving the way for affordable, decentralized sensors that support early diagnosis and better healthcare.
碱性磷酸酶（ALP）是一种存在于人体中的酶，负责使磷酸化的化学物质发生去磷酸化反应。它主要在骨骼、肝脏、肠道以及孕期的胎盘等器官中表达，在基因表达、转运和代谢等细胞过程中发挥着关键作用。生理状态下的碱性磷酸酶水平会因年龄和性别而有所不同，健康成年人的正常血清范围在40至190 U/L之间。碱性磷酸酶水平的变化可能表明多种病理状况，包括癌症的诊断和转移，以及骨骼生长功能障碍和低磷酸酯酶症。传统的碱性磷酸酶检测方法通常需要复杂的检测原理、大量的样品预处理以及专业的操作人员。在此，介绍了一种通过丝网印刷制备的便携式、柔性电化学传感器的研发，用于监测生物样品中的碱性磷酸酶水平。这种柔性电化学传感器具有高效、可持续、低成本和易于处理的特点，在缓冲溶液和人血清样品中的检测限分别低至0.03和0.08 U/L，并且具有低于10%的令人满意的重复性。这种简单的传感器配置方法能够实现疾病的实时监测，并改善即时诊断的可及性，为支持早期诊断和更好医疗保健的经济实惠、分散式传感器铺平了道路。
