Dentistry and nursing students experience significant anxiety, negatively impacting their well-being and academic performance. This study aims to assess the prevalence and relationships of stress, anxiety, depression, resilience, hope, and spiritual well-being among dentistry and nursing students, identify demographic influences and propose strategies to enhance resilience and well-being. This study surveyed 271 students attending Greece's departments of dentistry and nursing at the National and Kapodistrian University of Athens, using an electronic questionnaire aimed to assess stress, anxiety, and depression (depression, anxiety, stress scale-DASS-21); resilience (resilience assessment questionnaire-RAQ8, brief resilience scale-BRS); hope (adult hope scale-AHS); and spiritual well-being (functional assessment of chronic illness therapy-spiritual well-being scale-FACIT-Sp-12). The survey also collected demographic data to identify factors influencing these variables. Statistical analyses, including hierarchical multiple linear regression and -tests, were performed to analyze the relationships between variables. The sample included 145 dentistry and 126 nursing students, with 68.6% female and 80.1% undergraduate. Half of the students reported mild or higher levels of stress (48.7%), anxiety (51.3%), and depression (53.5%). The prevalence of depression was the highest in our sample, followed by anxiety and stress. Higher family wealth was associated with reduced stress levels, while female undergraduate students reported higher levels of anxiety than their male counterparts. Hope was a strong predictor of resilience, but stress and worry had a negative correlation. Promoting students' well-being and academic success requires effective stress-reduction and resilience-building techniques to improve students' performance and support future healthcare professionals' personal sustainability and holistic growth.