属于进化枝D β-碳酸酐酶的真菌羰基硫化物水解酶的特性分析
Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β-carbonic anhydrase.
作者信息
Iizuka Ryuka, Ogawa Takahiro, Tsukida Rikako, Noguchi Keiichi, Hibbett David, Katayama Yoko, Yoshida Makoto
机构信息
Department of Symbiotic Science of Environment and Natural Resources, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Graduate School of Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.
出版信息
FEBS Lett. 2025 Apr;599(8):1146-1158. doi: 10.1002/1873-3468.15098. Epub 2025 Jan 10.
Carbonyl sulfide hydrolase (COSase) is a unique enzyme that exhibits high activity towards carbonyl sulfide (COS) but low carbonic anhydrase (CA) activity, despite belonging to the CA family. COSase was initially identified in a sulfur-oxidizing bacterium and later discovered in the ascomycete Trichoderma harzianum strain THIF08. The COSase from T. harzianum has been recognized as a key enzyme in the assimilation of gaseous COS, and homologous genes are widely present not only in Ascomycota but also in Basidiomycota. Here, we characterized the COSases from the basidiomycete Gloeophyllum trabeum NBRC 6430 and T. harzianum to obtain detailed characteristics of fungal COSase. This study contributes to a better understanding of COS metabolism in fungi.
羰基硫化物水解酶（COSase）是一种独特的酶，尽管它属于碳酸酐酶（CA）家族，但对羰基硫化物（COS）具有高活性，而对碳酸酐酶的活性较低。COSase最初是在一种硫氧化细菌中发现的，后来在子囊菌哈茨木霉THIF08菌株中被发现。哈茨木霉的COSase被认为是气态COS同化过程中的关键酶，其同源基因不仅广泛存在于子囊菌门中，也存在于担子菌门中。在这里，我们对担子菌黄孢原毛平革菌NBRC 6430和哈茨木霉的COSase进行了表征，以获得真菌COSase的详细特性。这项研究有助于更好地理解真菌中的COS代谢。
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