Akhtardanesh Baharak, Mohammadi Elham, Sadr Soheil, Askari Asma, Tavakoli Zeinab Manzari, Ahmadi Rozhin, Nazemian Shakiba, Rashidi Hossein, Aghamiri Morteza, Golchin Mehdi, Imani Masoud
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Acta Trop. 2025 Feb;262:107521. doi: 10.1016/j.actatropica.2025.107521. Epub 2025 Jan 8.
Brucellosis is still a significant emerging threat to public health, as it can infect humans, wild, domestic animals, and livestock. Hence, the current study aims to determine the frequency of canine brucellosis (CB), its relationship with clinical findings and reproductive disorders in kennel and farm dogs, and its importance on public health.
From January 2022 to December 2023, a total of 150 blood samples were taken from 100 adult dogs in breeding kennels and 50 shepherd dogs in breeding farms in Kerman, Iran. Rose Bengal test (RBT) and Wright tests were used for the primary screening of Brucella abortus (B. abortus) and Brucella melitensis (B. melitensis) species, and conventional IS711-based PCR and real-time polymerase chain reaction (RT-PCR) were performed to determine the Brucella genus and species in all samples.
In kennel dogs, 34 % (34/100) had a history of breeding disorders, and 30 % (30/100) were RBT-positive. Moreover, B. canis and B. abortus species were detected in kennel dogs. Among farm dogs, 16 % (8/50) had a history of breeding disorders, and 28 % (14/50) were RBT positive. Additionally, B. canis, B. abortus, and B. melitensis species were detected in farm dogs. Feeding with unpasteurized milk was significantly related to a positive RBT in kennel dogs (p = 0.009), and there was a significant correlation between breeding disorders and seropositivity in kennel (p = 0.045) and farm dogs (p = 0.03).
The current study represents a significant advancement in understanding CB in Iran by the first molecular detection of B. canis, revealing B. melitensis and B. abortus as important pathogens in kennel and farm dogs and highlighting the public health significance of disease in Iran.
布鲁氏菌病仍是对公共卫生的重大新出现威胁，因为它可感染人类、野生动物、家畜和牲畜。因此，本研究旨在确定犬布鲁氏菌病（CB）的发生率、其与犬舍和农场犬临床症状及繁殖障碍的关系，以及其对公共卫生的重要性。
2022年1月至2023年12月，共从伊朗克尔曼的100只繁殖犬舍成年犬和50只繁殖农场牧羊犬采集了150份血液样本。采用玫瑰红试验（RBT）和wright试验对流产布鲁氏菌（B. abortus）和马尔他布鲁氏菌（B. melitensis）进行初步筛查，并对所有样本进行基于常规IS711的PCR和实时聚合酶链反应（RT-PCR）以确定布鲁氏菌属和种。
在犬舍犬中，34%（34/100）有繁殖障碍史，30%（30/100）RBT呈阳性。此外，在犬舍犬中检测到犬布鲁氏菌和流产布鲁氏菌。在农场犬中，16%（8/50）有繁殖障碍史，28%（14/50）RBT呈阳性。此外，在农场犬中检测到犬布鲁氏菌、流产布鲁氏菌和马尔他布鲁氏菌。用未巴氏消毒牛奶喂养与犬舍犬RBT阳性显著相关（p = 0.009），繁殖障碍与犬舍（p = 0.045）和农场犬（p = 0.03）血清阳性之间存在显著相关性。
本研究通过首次对犬布鲁氏菌进行分子检测，在理解伊朗犬布鲁氏菌病方面取得了重大进展，揭示了马尔他布鲁氏菌和流产布鲁氏菌是犬舍和农场犬的重要病原体，并突出了该病在伊朗的公共卫生意义。
