组蛋白H5对鸟类红细胞系分化过程中单核小体结构的影响。
Influence of histone H5 on mononucleosome structure during differentiation in the avian erythroid series.
作者信息
Haye K R, Schlegel R A
出版信息
Exp Cell Res. 1985 Apr;157(2):504-10. doi: 10.1016/0014-4827(85)90135-1.
Changes in nucleosome repeat length during avian erythroid development have been previously correlated with changes in H5 content. In order to determine the effects of H5 on the length of DNA in mononucleosomal particles as a function of differentiation, a two-dimensional electrophoretic system was used to analyse DNA and histones of particles generated by micrococcal nuclease digestion of nuclei from several stages of erythroid development. Although the relative proportions of H5- to H1-containing mononucleosomes increased during development, only in mature erythrocytes did H5 protect a greater length of linker DNA from micrococcal nuclease digestion than did H1. These results suggest that changes in average nucleosome repeat length during erythroid development can be attributed only partially to an increase in the proportion of H5-containing nucleosomes which contribute to this average.
鸟类红细胞发育过程中核小体重复长度的变化先前已与H5含量的变化相关联。为了确定H5对单核小体颗粒中DNA长度的影响（作为分化的函数），使用二维电泳系统分析了来自红细胞发育几个阶段的细胞核经微球菌核酸酶消化产生的颗粒的DNA和组蛋白。尽管在发育过程中含H5的单核小体与含H1的单核小体的相对比例增加，但只有在成熟红细胞中，H5才比H1保护更长的连接DNA免受微球菌核酸酶消化。这些结果表明，红细胞发育过程中平均核小体重复长度的变化只能部分归因于含H5的核小体比例的增加，而含H5的核小体对该平均值有贡献。
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