含有组蛋白H1和H5的核小体的分离。

Separation of nucleosomes containing histones H1 and H5.

作者信息

Bakayeva T G, Bakayev V V

出版信息

Mol Biol Rep. 1978 Oct 16;4(3):185-9. doi: 10.1007/BF00777522.

Abstract

Hen erythrocyte chromatin was digested with staphylococcal nuclease and fractionated by electrophoresis in polyacrylamide gels. Instead of the three bands described for mouse carcinoma chromatin, four main discrete components (MN1, MN2, MN2E and MN3) were resolved in the mononucleosome fraction of erythrocyte chromatin. MN2 contained all five histones and a DNA fragment of 165--180 base pairs. MN2E comprised four nucleosomal histones plus histone H5 (but not H1) and a DNA fragment of 170--190 base pairs. The relatively nuclease resistant MN3 fraction of erythrocyte nucleosomes contained H1 but no H5 histone. A more accurate analysis of the MN2 fraction in mouse carcinoma nucleosomes revealed some additional microheterogeneity depending on the presence of two different subfractions of H1.

摘要

用葡萄球菌核酸酶消化鸡红细胞染色质，并在聚丙烯酰胺凝胶中进行电泳分级分离。在红细胞染色质的单核小体部分，分离出的不是小鼠癌染色质所描述的三条带，而是四个主要的离散组分（MN1、MN2、MN2E和MN3）。MN2包含所有五种组蛋白和一个165 - 180个碱基对的DNA片段。MN2E由四种核小体组蛋白加组蛋白H5（但不含H1）和一个170 - 190个碱基对的DNA片段组成。红细胞核小体相对抗核酸酶的MN3部分含有H1但不含H5组蛋白。对小鼠癌核小体中MN2部分进行更精确的分析发现，根据H1的两种不同亚组分的存在，存在一些额外的微异质性。