Yu Lihuai, Qiu Guangzhi, Yu Xiaomu, Zhao Jianwei, Liu Jun, Wang Hongrong, Dong Li
College of Animal Science and Technology, Yangzhou University, Yangzhou 215009, China.
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
Animals (Basel). 2024 Dec 24;15(1):9. doi: 10.3390/ani15010009.
The aim of this study was to investigate the effects of terpinen-4-ol (TER) supplementation on the intestinal barrier function of pigs. Five groups of fifty 28-day-old piglets with comparable body weights were randomly assigned to the following groups: the control group (CON), the lipopolysaccharide group (LPS), the low TER group (PLT), the middle TER group (PMT), and the high TER group (PHT). The basal diet was given to the CON and LPS groups, and 30, 60, or 90 mg/kg TER was added to the basal diet for the TER groups. After the 21-day trial period, piglets in the LPS and TER groups received an intraperitoneal injection of 100 μg/kg body weight of LPS, whereas the piglets in the CON group received an injection of 0.9% normal saline solution. The results showed that LPS stimulation resulted in a decrease ( < 0.05) in the depth of colonic crypts in piglets, which was greater ( < 0.05) in the TER group. Compared with those in the CON group, the number of goblet cells and MUC2 expression were decreased in the colon of piglets in the LPS group, while these parameters were increased in the PMT group ( < 0.05). The malondialdehyde (MDA) content was greater in the colon of the LPS group than in that of the CON group, while the activities of glutathione peroxidase (), superoxide dismutase (), and catalase () were lower in the colon of the LPS group; conversely, the MDA content was lower in the colons of the PLT and PMT groups than in those of the LPS group ( < 0.05). TER also reduced ( < 0.05) LPS-induced upregulation of and expression, along with the relative gene expression of , , and in the colon of piglets ( < 0.05). Compared with those in the CON group, the abundances of and in the LPS group were lower ( < 0.05), and those in the TER group were significantly greater than those in the LPS group. Compared with those in the CON group, the abundance of in the LPS group increased ( < 0.05), while the abundance of and increased ( < 0.05) in the colon of the PHT group compared with that in the LPS group. In conclusion, TER effectively improved the intestinal barrier function of the colon in weaning piglets. Based on the results of this study, the appropriate dose of TER in the diets of weaning piglets was 60 mg/kg.
本研究旨在探讨补充松油烯 -4-醇（TER）对仔猪肠道屏障功能的影响。将五组体重相当的50头28日龄仔猪随机分为以下几组：对照组（CON）、脂多糖组（LPS）、低TER组（PLT）、中TER组（PMT）和高TER组（PHT）。CON组和LPS组给予基础日粮，TER组在基础日粮中添加30、60或90 mg/kg TER。经过21天的试验期后，LPS组和TER组的仔猪腹腔注射100 μg/kg体重的LPS，而CON组的仔猪注射0.9%的生理盐水溶液。结果表明，LPS刺激导致仔猪结肠隐窝深度降低（<0.05），TER组降低幅度更大（<0.05）。与CON组相比，LPS组仔猪结肠中杯状细胞数量和MUC2表达降低，而PMT组这些参数增加（<0.05）。LPS组结肠中丙二醛（MDA）含量高于CON组，而LPS组结肠中谷胱甘肽过氧化物酶（）、超氧化物歧化酶（）和过氧化氢酶（）的活性较低；相反，PLT组和PMT组结肠中MDA含量低于LPS组（<0.05）。TER还降低了（<0.05）LPS诱导的仔猪结肠中 和 表达上调以及 、 和 的相对基因表达（<0.05）。与CON组相比，LPS组中 和 的丰度较低（<0.05），TER组显著高于LPS组。与CON组相比，LPS组中 的丰度增加（<0.05），而与LPS组相比，PHT组结肠中 和 的丰度增加（<0.05）。总之，TER有效改善了断奶仔猪结肠的肠道屏障功能。基于本研究结果，断奶仔猪日粮中TER的适宜剂量为60 mg/kg。
