El Brini Zineb, Fassi Fihri Ouafaa, Paillot Romain, Lotfi Chafiqa, Amraoui Farid, El Ouadi Hanane, Dehhaoui Mohamed, Colitti Barbara, Alyakine Hassan, Piro Mohammed
Department of Medicine, Surgery, and Reproduction, Agronomy and Veterinary Institute Hassan II, Rabat 10000, Morocco.
Department of Microbiology, Immunology and Contagious Diseases, Agronomy and Veterinary Institute Hassan II, Rabat 10000, Morocco.
Animals (Basel). 2021 Sep 29;11(10):2851. doi: 10.3390/ani11102851.
This study reports the first equine herpesvirus-1 (EHV-1) and equine herpesvirus-4 (EHV-4) seroprevalence investigation in horse populations of Morocco in 24 years. It also aims to determine antibody titers in horses vaccinated under field conditions with a monovalent EHV-1 vaccine. Blood samples were collected from 405 horses, including 163 unvaccinated and 242 vaccinated animals. They were tested using a commercial type-specific enzyme-linked immunosorbent assay (ELISA) and a virus neutralization test (VNT). Overall, 12.8% unvaccinated, and 21.8% vaccinated horses were positive for EHV-1. All samples were positive for EHV-4 when tested with the type-specific ELISA. In the vaccinated group, the VNT revealed a mean antibody titer of 1:49 for EHV-1 and 1:45 for EHV-4. The present study demonstrates that EHV-1 and EHV-4 are endemic in the horse populations in the north of Morocco, with prevalence differences between regions. Furthermore, horses vaccinated with a monovalent EHV-1 vaccine had low antibodies titers. This study highlights the necessity to establish and/or support efficient biosecurity strategies based on sound management of horses and characterize further and potentially improve the efficiency of the EHV vaccines and vaccination protocol used in the field.
本研究报告了24年来摩洛哥马群中首次进行的马疱疹病毒1型（EHV-1）和马疱疹病毒4型（EHV-4）血清流行率调查。它还旨在确定在野外条件下接种单价EHV-1疫苗的马匹中的抗体滴度。从405匹马中采集血样，包括163匹未接种疫苗的和242匹接种疫苗的动物。使用商业型特异性酶联免疫吸附测定（ELISA）和病毒中和试验（VNT）对它们进行检测。总体而言，12.8%的未接种疫苗马匹和21.8%的接种疫苗马匹EHV-1呈阳性。当用型特异性ELISA检测时，所有样本EHV-4均呈阳性。在接种疫苗的组中，VNT显示EHV-1的平均抗体滴度为1:49，EHV-4为1:45。本研究表明，EHV-1和EHV-4在摩洛哥北部的马群中呈地方流行，不同地区患病率存在差异。此外，接种单价EHV-1疫苗的马匹抗体滴度较低。本研究强调了基于合理的马匹管理建立和/或支持有效的生物安全策略的必要性，并进一步表征和潜在提高野外使用的EHV疫苗和接种方案的效率。
