Guo Wei, Yan Shiqiang, Xiao Chaoqiang, Shi Dayong, Hua Qing, Hao Xiaowen, Zhang Wenjuan, Zhuang Xuming
Shandong Dyne Marine Biopharmaceutical Co., Ltd., Weihai 264300, China.
School of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
Molecules. 2025 Jan 2;30(1):152. doi: 10.3390/molecules30010152.
Oseltamivir is a drug that has been widely used to prevent and treat influenza A and B. In this work, an ultrasensitive, simple, and novel electrochemiluminescence (ECL) sensor combined with molecularly imprinted polymers (MIP-ECL) based on a graphene-like two-dimensional material, Mxene quantum dots (MQDs) was constructed to selectively detect oseltamivir. A molecularly imprinted polymer membrane containing an oseltamivir template was constructed by electropolymerization and elution of modified MQDs on a glassy carbon electrode. Under optimized experimental conditions, the MIP-ECL sensor could detect oseltamivir in the range of 10 to 10 M (R = 0.9816), with a low limit of detection of 6.5 × 10 M (S/N = 3), and the recovery rates of oseltamivir in biological samples were 92.21-104.2%, with relative standard deviations of 3.70%~5.70%. The developed MIP-ECL sensor provides a new idea for detecting oseltamivir, which was successfully applied to the determination of oseltamivir in serum samples, indicating great potential for application in clinical diagnostics.
奥司他韦是一种已被广泛用于预防和治疗甲型和乙型流感的药物。在这项工作中，基于类石墨烯二维材料碳化钛纳米片量子点（MQDs）构建了一种超灵敏、简单且新颖的结合分子印迹聚合物的电化学发光（ECL）传感器（MIP-ECL），用于选择性检测奥司他韦。通过在玻碳电极上对修饰的MQDs进行电聚合和洗脱，构建了含有奥司他韦模板的分子印迹聚合物膜。在优化的实验条件下，MIP-ECL传感器能够检测浓度范围为10至10 M的奥司他韦（R = 0.9816），检测限低至6.5×10 M（S/N = 3），生物样品中奥司他韦的回收率为92.21 - 104.2%，相对标准偏差为3.70%至5.70%。所开发的MIP-ECL传感器为检测奥司他韦提供了新思路，已成功应用于血清样品中奥司他韦的测定，显示出在临床诊断中具有巨大的应用潜力。
