Li Mingxuan, Li Zhenrun, Wang Peilin, Ma Qiang
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Biosens Bioelectron. 2023 May 15;228:115225. doi: 10.1016/j.bios.2023.115225. Epub 2023 Mar 11.
In this work, a novel ECL biosensor has been developed based on bimetallic MXene derivative QDs (MoTiC QDs) and SnS nanosheets/lipid bilayer to detect the gastric cancer marker miRNA-27a-3p. On the one hand, the inter-band excitation of MoTiC QDs can inject the additional carriers, which were less suppressed by boundary effects and made a significant contribution to the luminescence process. Semiconductor MoTiC further inhibited the formation of internal electric field and potential oxidation. Therefore, MoTiC QDs processed superior luminous intensity and better stability. On the other hand, SnS nanosheets coated with phospholipid bilayer were designed as sensing interface. SnS nanosheets not only enhanced the luminous intensity of MoTiC QDs by virtue of their large surface area and low dielectric constant, but also improved the stability of lipid bilayer. Due to the excellent properties and synergy work of MoTiC QDs and the lipid bilayer-modified SnS nanosheets, the sensing system displayed high sensitivity and good reproducibility in the analysis application. As a result, the biosensor displayed good linear correlation between the ECL intensity and the concentration of miRNA-27a-3p over a wide range from 1 fM to 10 nM with the detection limit as low as 1 fM. The sensing system including the joint contribution of MoTiC QDs, SnS nanosheets and lipid bilayers had great potential for clinical applications.
在这项工作中，基于双金属MXene衍生物量子点（MoTiC量子点）和SnS纳米片/脂质双层开发了一种新型的电化学发光（ECL）生物传感器，用于检测胃癌标志物miRNA - 27a - 3p。一方面，MoTiC量子点的带间激发可以注入额外的载流子，这些载流子受边界效应的抑制较小，对发光过程有显著贡献。半导体MoTiC进一步抑制了内部电场的形成和潜在的氧化。因此，MoTiC量子点具有优异的发光强度和更好的稳定性。另一方面，将涂有磷脂双层的SnS纳米片设计为传感界面。SnS纳米片不仅凭借其大表面积和低介电常数增强了MoTiC量子点的发光强度，还提高了脂质双层的稳定性。由于MoTiC量子点和脂质双层修饰的SnS纳米片的优异性能和协同作用，该传感系统在分析应用中表现出高灵敏度和良好的重现性。结果，该生物传感器在1 fM至10 nM的宽范围内，ECL强度与miRNA - 27a - 3p浓度之间呈现良好的线性相关性，检测限低至1 fM。包括MoTiC量子点、SnS纳米片和脂质双层共同作用的传感系统在临床应用中具有巨大潜力。
