Yang Guotang, Han Yi, Yin Huihui, Li Xingfeng, Wang Honggang, Bao Yinguang
State Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai'an 271018, China.
Agronomy College, Shandong Agricultural University, Tai'an 271018, China.
Plants (Basel). 2024 Dec 25;14(1):27. doi: 10.3390/plants14010027.
Stripe rust, induced by f. sp. (), is one of the most destructive fungal diseases of wheat worldwide. , a significant wild relative for wheat improvement, exhibits innate immunity to this disease. To transfer the stripe rust resistance gene from to wheat, two translocation lines, SN21171 and SN52684, were produced through distant hybridization techniques. Disease evaluation results showed that these two lines were immune to species CYR32 at the adult plant stage. Molecular cytogenetic analyses and specific intron-targeting markers amplification results revealed that SN21171 and SN52684 harbor several T3E-3DS·3DL and T1E-1BS·1BL translocation chromosomes. Furthermore, the comparison of the chromosome karyotype from two translocation lines and their recurrent parent YN15, revealed that structural variation occurred in chromosomes 2A, 5A, 2B, 4B, 5B, and 6B in SN21171 and chromosomes 5A, 3B, 4B, 5B, 6B, and 7B in SN52684. Agronomic trait assessments uncovered advantageous properties in both lines, with SN21171 matching the recurrent parent and SN52684 exhibiting elevated higher grain number per main spike and increased thousand grain weight. These two translocation lines and specific markers may apply to wheat stripe rust-resistance breeding.
条锈病由小麦条锈菌（Puccinia striiformis f. sp. tritici）引起，是全球范围内对小麦最具毁灭性的真菌病害之一。长穗偃麦草（Thinopyrum elongatum）是小麦改良的重要野生近缘种，对这种病害表现出天然免疫。为了将长穗偃麦草的抗条锈病基因转移到小麦中，通过远缘杂交技术培育了两个易位系SN21171和SN52684。病害评估结果表明，这两个品系在成株期对条锈菌CYR32小种免疫。分子细胞遗传学分析和特定内含子靶向标记扩增结果显示，SN21171和SN52684含有多个T3E-3DS·3DL和T1E-1BS·1BL易位染色体。此外，对两个易位系及其轮回亲本YN15的染色体核型比较表明，SN21171的2A、5A、2B、4B、5B和6B染色体以及SN52684的5A、3B、4B、5B、6B和7B染色体发生了结构变异。农艺性状评估发现这两个品系都具有优良特性，SN21171与轮回亲本相似，SN52684的主穗粒数更多，千粒重增加。这两个易位系和特定标记可应用于小麦抗条锈病育种。
