National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Zhoucun District Agricultural Technology Service Center, Zibo, Shandong, 255300, China.
Nat Commun. 2023 Jul 19;14(1):4353. doi: 10.1038/s41467-023-39993-2.
Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), can significantly affect wheat production. Cloning resistance genes is critical for efficient and effective breeding of stripe rust resistant wheat cultivars. One resistance gene (Yr10) underlying the Pst resistance locus Yr10 has been cloned. However, following haplotype and linkage analyses indicate the presence of additional Pst resistance gene(s) underlying/near Yr10 locus. Here, we report the cloning of the Pst resistance gene YrNAM in this region using the method of sequencing trait-associated mutations (STAM). YrNAM encodes a non-canonical resistance protein with a NAM domain and a ZnF-BED domain. We show that both domains are required for resistance. Transgenic wheat harboring YrNAM gene driven by its endogenous promoter confers resistance to stripe rust races CYR32 and CYR33. YrNAM is an ancient gene and present in wild wheat species Aegilops longissima and Ae. sharonensis; however, it is absent in most wheat cultivars, which indicates its breeding value.
条锈病（Yellow Rust），由条形柄锈菌小麦专化型（Puccinia striiformis f. sp. tritici，Pst）引起，会对小麦生产造成严重影响。克隆抗病基因对于培育抗条锈病小麦品种的高效和有效育种至关重要。已有一个位于 Yr10 位点的抗病基因（Yr10）被克隆。然而，单倍型和连锁分析表明，在 Yr10 位点的下游/附近还存在其他的 Pst 抗性基因。在这里，我们使用测序性状相关突变（Sequencing Trait-Associated Mutations，STAM）的方法，报道了在该区域克隆到的 Pst 抗性基因 YrNAM。YrNAM 编码一种具有 NAM 结构域和 ZnF-BED 结构域的非典型抗性蛋白。我们证明这两个结构域都是抗性所必需的。由其内源启动子驱动的转 YrNAM 基因的小麦对条锈病小种 CYR32 和 CYR33 表现出抗性。YrNAM 是一个古老的基因，存在于野生小麦物种长穗偃麦草和节节麦中；然而，它在大多数小麦品种中缺失，这表明它具有很高的育种价值。
