Jerzemowska Grażyna, Podlacha Magdalena, Orzeł-Gryglewska Jolanta
Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, 59 Wita Stwosza Str., 80-308 Gdansk, Poland.
Department of Molecular Biology, Faculty of Biology, University of Gdansk, 59 Wita Stwosza Str., 80-308 Gdansk, Poland.
Int J Mol Sci. 2024 Dec 28;26(1):182. doi: 10.3390/ijms26010182.
Amphetamine abuse is a global health epidemic that is difficult to treat due to individual differences in response to environmental factors, including stress reactivity and anxiety levels, as well as individual neuronal differences, which may result in increased/decreased vulnerability to addiction. In the present study, we investigated whether the Wistar rats behavioral traits of high (HR) and low (LR) locomotor activity to novelty influence motivational behavior (induced feeding model; iFR by electrical stimulation of the ventral tegmental area; Es-VTA) supported by amphetamine injection into the nucleus accumbens shell (AcbSh) (HR, n = 5; LR, n = 5). A correlation was found between the novelty test's locomotor activity score and the frequency threshold percentage change ( < 0.001, Rs = -0.867). In HR, there was a shortening (-24.16%), while in LR, there was a lengthening (+51.84%) of iFR latency. Immunofluorescence studies showed differential neuronal density (activity of tyrosine hydroxylase, choline acetyltransferase, and cFos protein) in the selected brain structures in HR and LR animals as well as in comparison to a control group (HR, n = 5; LR, n = 5). These results contribute to expanding the state of knowledge of the behavioral and neuronal propensity to take drug abuse.
苯丙胺滥用是一种全球性的健康流行病，由于个体对环境因素（包括应激反应性和焦虑水平）的反应存在差异，以及个体神经元差异，导致其难以治疗，这些差异可能会增加/降低成瘾易感性。在本研究中，我们调查了对新奇事物具有高（HR）和低（LR）运动活性的Wistar大鼠的行为特征是否会影响由向伏隔核壳（AcbSh）注射苯丙胺所支持的动机行为（诱导进食模型；通过电刺激腹侧被盖区进行诱导式食物奖励；Es-VTA）（HR组，n = 5；LR组，n = 5）。我们发现新奇性测试的运动活性得分与频率阈值百分比变化之间存在相关性（<0.001，Rs = -0.867）。在HR组中，诱导式食物奖励潜伏期缩短了（-24.16%），而在LR组中则延长了（+51.84%）。免疫荧光研究显示，与对照组（HR组，n = 5；LR组，n = 5）相比，HR组和LR组动物选定脑结构中的神经元密度（酪氨酸羟化酶、胆碱乙酰转移酶和cFos蛋白的活性）存在差异。这些结果有助于扩展对药物滥用行为和神经元倾向的认识。
