Ruciński Jan, Kurowska-Rucińska Ewelina, Myślińska Dorota, Grembecka Beata, Piekarczyk Natalia, Necel Agnieszka, Kosznik-Kwaśnicka Katarzyna, Majkutewicz Irena
Department of Animal and Human Physiology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59 St., 80-308 Gdańsk, Poland.
Division of Medical Microbiology, Department of Microbiology, Faculty of Medicine, Medical University of Gdańsk, M. Skłodowskiej-Curie 3a St., 80-210 Gdańsk, Poland.
Int J Mol Sci. 2025 May 3;26(9):4353. doi: 10.3390/ijms26094353.
The amygdala, especially its central nucleus (CeA), is one of the key brain structures regulating fear, anxiety and stress responses and is also involved in gut microbiota signal processing. Amygdala hyperactivity, as well as microbiota alterations, plays an important role in the pathophysiology of anxiety disorders, depression or post-traumatic stress disorder (PTSD). The present study determines whether 3 weeks of galactooligosaccharide (GOS) supplementation alleviates behavioural, haematological, immunological and gut microbiota disturbances induced by long-term electrical stimulation of the CeA in rats (Stim). The unsupplemented Stim group showed locomotor hyperactivity and higher anxiety (measured with an actometer and the elevated plus maze, respectively), as well as a decrease in white blood cells (WBCs), lymphocytes (LYMs), red blood cells (RBCs) and platelets (PLTs); an elevation of TNFα; a reduction in IL-10 concentration in plasma; and microbiota alterations as compared to the control (Sham) group. GOS supplementation alleviated all these Stim-induced adverse effects or even normalised them to the sham group level. The effect of GOS was comparable to citalopram and even more effective in WBC and PLT normalisation and IL-10 induction. The obtained results indicate the high therapeutic potential of GOS in anxiety and stress-related disorders. GOS supplementation may support conventional therapy or the prevention of PTSD, depression and anxiety disorders.
杏仁核，尤其是其中央核（CeA），是调节恐惧、焦虑和应激反应的关键脑结构之一，也参与肠道微生物群信号处理。杏仁核功能亢进以及微生物群改变在焦虑症、抑郁症或创伤后应激障碍（PTSD）的病理生理学中起重要作用。本研究确定补充3周低聚半乳糖（GOS）是否能减轻大鼠长期电刺激CeA（刺激组）所诱发的行为、血液学、免疫学和肠道微生物群紊乱。未补充GOS的刺激组表现出运动亢进和更高的焦虑水平（分别用活动计和高架十字迷宫测量），以及白细胞（WBC）、淋巴细胞（LYM）、红细胞（RBC）和血小板（PLT）减少；肿瘤坏死因子α（TNFα）升高；血浆中白细胞介素-10（IL-10）浓度降低；与对照组（假手术组）相比，微生物群发生改变。补充GOS可减轻所有这些由刺激诱发的不良反应，甚至使其恢复到假手术组水平。GOS的效果与西酞普兰相当，在使白细胞和血小板恢复正常以及诱导白细胞介素-10方面甚至更有效。所得结果表明GOS在焦虑和应激相关疾病中具有很高的治疗潜力。补充GOS可能有助于传统疗法或预防创伤后应激障碍、抑郁症和焦虑症。
