Hui Qianlong, Song Ting, Yang Dantong, Wu Qibin, Guo Jinlong, Que Youxiong, Xu Liping
National Engineering Research Center for Sugarcane, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology/Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China.
Int J Mol Sci. 2024 Dec 30;26(1):226. doi: 10.3390/ijms26010226.
Sugarcane ( spp.) is globally considered an important crop for sugar and biofuel production. During sugarcane production, the heavy reliance on chemical nitrogen fertilizer has resulted in low nitrogen use efficiency (NUE) and high loss. Up until now, there has been extensive research on the transcriptomic dynamics during sugarcane response to low nitrogen (LN) stress. However, the specific contribution of to the NUE of modern sugarcane remains unclear. In the present study, the comparative transcriptome analysis of two contrasting sugarcane cultivars in response to nitrogen deficiency was performed via the combination of genomes of and . Sub-genome analysis indicated that supports the high NUE of modern sugarcane by providing genes related to nitrogen and carbohydrate metabolism, photosynthesis, and amino acid metabolism. Additionally, the key genes involved in nitrogen metabolism from the were successfully identified through weighted gene co-expression network analyses (WGCNA), and a high-affinity nitrate transporter named was subsequently cloned. Heterogeneous expression of the , a cell membrane-localized protein, could enhance the growth of under low nitrate conditions. Furthermore, a conserved protein module known as NAR2.1/NRT2.3 was shown to regulate the response to LN stress in sugarcane roots through molecular interaction. This work helps to clarify the contribution of to the NUE in modern sugarcane, and the function of the in sugarcane.
甘蔗（spp.）在全球范围内被视为用于制糖和生物燃料生产的重要作物。在甘蔗生产过程中，对化学氮肥的严重依赖导致了低氮利用效率（NUE）和高损失。到目前为止，已经对甘蔗响应低氮（LN）胁迫期间的转录组动态进行了广泛研究。然而，其对现代甘蔗NUE的具体贡献仍不清楚。在本研究中，通过结合 和 的基因组，对两个对比甘蔗品种响应氮缺乏进行了比较转录组分析。亚基因组分析表明， 通过提供与氮和碳水化合物代谢、光合作用以及氨基酸代谢相关的基因，支持了现代甘蔗的高NUE。此外，通过加权基因共表达网络分析（WGCNA）成功鉴定了来自 的参与氮代谢的关键基因，随后克隆了一个名为 的高亲和力硝酸盐转运蛋白。 （一种定位于细胞膜的蛋白质）的异源表达可以增强 在低硝酸盐条件下的生长。此外，一个名为NAR2.1/NRT2.3的保守蛋白质模块通过分子相互作用被证明可调节甘蔗根对LN胁迫的响应。这项工作有助于阐明 对现代甘蔗NUE的贡献以及 在甘蔗中的功能。
