Li Hang, Liu Li, Kong Xianhui, Wang Xuwen, Si Aijun, Zhao Fuxiang, Huang Qian, Yu Yu, Chen Zhiwen
Cotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, China.
Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education, Key Laboratory of Graphene Forestry Application of National Forest and Grass Administration, Shanxi Datong University, Datong 037009, China.
Int J Mol Sci. 2025 Jan 2;26(1):329. doi: 10.3390/ijms26010329.
Salt stress is an environmental factor that limits plant seed germination, growth, and survival. We performed a comparative RNA sequencing transcriptome analysis during germination of the seeds from two cultivars with contrasting salt tolerance responses. A transcriptomic comparison between salt-tolerant cotton cv Jin-mian 25 and salt-sensitive cotton cv Su-mian 3 revealed both similar and differential expression patterns between the two genotypes during salt stress. The expression of genes related to aquaporins, kinases, reactive oxygen species (ROS) scavenging, trehalose biosynthesis, and phytohormone biosynthesis and signaling that include ethylene (ET), gibberellin (GA), abscisic acid (ABA), jasmonic acid (JA), and brassinosteroid (BR) were systematically investigated between the cultivars. Despite the involvement of these genes in cotton's response to salt stress in positive or negative ways, their expression levels were mostly similar in both genotypes. Interestingly, a gene () was identified, which encodes a leucine-rich repeat receptor-like protein kinase (LRR-RLK). This gene showed an induced expression pattern after salt stress treatment in salt-tolerant cv Jin-mian 25 but not salt-sensitive cv Su-mian 3. Our multifaceted transcriptome approach illustrated a differential response to salt stress between salt-tolerant and salt-sensitive cotton.
盐胁迫是一种限制植物种子萌发、生长和存活的环境因素。我们对两个具有不同耐盐性响应的棉花品种种子萌发过程进行了比较RNA测序转录组分析。耐盐棉花品种晋棉25和盐敏感棉花品种苏棉3之间的转录组比较揭示了两种基因型在盐胁迫期间相似和差异的表达模式。在这两个品种之间系统地研究了与水通道蛋白、激酶、活性氧（ROS）清除、海藻糖生物合成以及包括乙烯（ET）、赤霉素（GA）、脱落酸（ABA）、茉莉酸（JA）和油菜素内酯（BR）在内的植物激素生物合成和信号传导相关基因的表达。尽管这些基因以正向或负向方式参与棉花对盐胁迫的响应，但它们在两种基因型中的表达水平大多相似。有趣的是，鉴定出了一个基因（），其编码富含亮氨酸重复序列的类受体蛋白激酶（LRR-RLK）。该基因在耐盐品种晋棉25中经盐胁迫处理后呈现诱导表达模式，而在盐敏感品种苏棉3中则未出现。我们多方面的转录组方法阐明了耐盐和盐敏感棉花对盐胁迫的差异响应。
