Iannuzzo Fortuna, Schiano Elisabetta, Maisto Maria, Schettino Anna, Marigliano Noemi, Saviano Anella, Abo Mansour Adel, Iqbal Asif Jilani, Maione Francesco, Tenore Gian Carlo, Novellino Ettore
Department of Pharmacy, University of Chieti-Pescara G. D'Annunzio, 66100 Chieti, Italy.
Inventia Biotech-Healthcare Food Research Center s.r.l., Strada Statale Sannitica KM 20.700, 81020 Caserta, Italy.
Nutrients. 2024 Dec 28;17(1):73. doi: 10.3390/nu17010073.
BACKGROUND/OBJECTIVES: Benign prostatic hyperplasia (BPH) is one of the most common chronic diseases affecting the urinary tract that occurs mainly in men over 40 years of age. Among the natural therapies, proanthocyanidins (PACs), which can treat a wide range of immune-mediated inflammatory diseases (IMIDs), have been shown to play an important role in the treatment of pathologies concerning prostate health. In this regard, the present study aimed to evaluate the different bioactivities of a grape seed extract (GSE), rich in polymeric PACs, and its version processed under alkaline conditions (ATGSE), characterized by a higher content of oligomeric PACs, in an animal model of BPH induced by subcutaneous injection of testosterone (1 mg/mouse).
These latter were divided into a control group (vehicle, olive oil), a BPH group (testosterone 1 mg/mouse), and four treatment groups treated with GSE (500 mg/kg) and ATGSE (125, 250, 500 mg/kg) by oral gavage. At the experimental endpoint (4 weeks), hematological and biochemical analyses of blood and tissues were performed.
Data showed that oral administration of ATGSE (250 mg/kg) was significantly more effective than GSE in reducing prostate ( ≤ 0.0001) and seminal vesicle ( ≤ 0.0001) weight. Moreover, ATGSE exhibited enhanced effectiveness in significantly reducing PSA levels ( ≤ 0.0001 vs. GSE) and the expression of key pro-inflammatory cyto-chemokines in prostate and seminal vesicles homogenates.
These findings pave the way for the clinical application of ATGSE as a nutraceutical and/or functional food.
背景/目的：良性前列腺增生（BPH）是影响尿路的最常见慢性疾病之一，主要发生在40岁以上男性中。在自然疗法中，原花青素（PACs）可治疗多种免疫介导的炎症性疾病（IMIDs），已被证明在前列腺健康相关疾病的治疗中发挥重要作用。在这方面，本研究旨在评估富含聚合PACs的葡萄籽提取物（GSE）及其在碱性条件下加工的产品（ATGSE，其特征为低聚PACs含量较高）在皮下注射睾酮（1mg/小鼠）诱导的BPH动物模型中的不同生物活性。
将后者分为对照组（赋形剂，橄榄油）、BPH组（睾酮1mg/小鼠）以及四个用GSE（500mg/kg）和ATGSE（125、250、500mg/kg）通过灌胃给药的治疗组。在实验终点（4周）时，对血液和组织进行血液学和生化分析。
数据显示，口服ATGSE（250mg/kg）在减轻前列腺重量（≤0.0001）和精囊重量（≤0.0001）方面比GSE显著更有效。此外，ATGSE在显著降低PSA水平（与GSE相比≤0.0001）以及前列腺和精囊匀浆中关键促炎细胞因子的表达方面表现出更强的效果。
这些发现为ATGSE作为营养保健品和/或功能性食品的临床应用铺平了道路。
