Mance Kristan Romana, Jurgec Staša, Potočnik Uroš, Marhl Marko, Gašperšič Rok
Community Health Centre dr. Adolf Drolc Maribor, Ulica talcev 9, 2000 Maribor, Slovenia.
Department of Periodontology, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
J Clin Med. 2024 Dec 25;14(1):24. doi: 10.3390/jcm14010024.
: Periodontitis is an inflammatory disease induced by bacteria in dental plaque that can activate the host's immune-inflammatory response and invade the bloodstream. We hypothesized that a higher periodontal inflamed surface area (PISA) is associated with higher levels of inflammatory biomarkers, lower levels of antioxidants, and mitochondrial DNA copy number (mtDNAcn). : Using periodontal parameters, we calculated the PISA score, measured the levels of inflammatory biomarkers and antioxidants in the serum, and took buccal swabs for mtDNA and nuclear DNA (nDNA) extraction. : Higher PISA was associated with higher CRP levels, higher leukocyte, neutrophil, and erythrocyte counts, and lower magnesium-to-calcium ratio, but not with mtDNAcn. A higher number of deep pockets was associated with higher leukocytes and neutrophil counts and higher uric acid levels. : The PISA score might be an appropriate parameter to assess the inflammatory burden of periodontitis, but not to assess mitochondrial dysfunction after mtDNA isolation from buccal swabs.
牙周炎是一种由牙菌斑中的细菌引发的炎症性疾病，它能够激活宿主的免疫炎症反应并侵入血液循环。我们推测，较高的牙周炎症表面积（PISA）与较高水平的炎症生物标志物、较低水平的抗氧化剂以及线粒体DNA拷贝数（mtDNAcn）相关。：利用牙周参数，我们计算了PISA评分，测定了血清中炎症生物标志物和抗氧化剂的水平，并采集颊拭子用于提取mtDNA和核DNA（nDNA）。：较高的PISA与较高的CRP水平、较高的白细胞、中性粒细胞和红细胞计数以及较低的镁钙比相关，但与mtDNAcn无关。较深牙周袋的数量较多与较高的白细胞和中性粒细胞计数以及较高的尿酸水平相关。：PISA评分可能是评估牙周炎炎症负担的一个合适参数，但从颊拭子中分离mtDNA后，它并非评估线粒体功能障碍的合适参数。
