Diffusion of fluorescein in the corneal stroma.

Movement of fluorescein in the eye was simulated on the basis of a multiple-compartment model, in which the cornea was divided into a series of cylindrical sleeves to allow 3-dimensional diffusion of fluorescein with rapid exchange at the limbus. If the dye was administered into the central 5 mm of the cornea, the loss at the limbus was found to be negligible in the early period but it became considerable later. The effect of fluorescein exchange at the limbus on the calculations of the dye transfer coefficients was estimated. Systematic errors in the fluorophotometric measurement of aqueous humor flow are discussed.