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从不同方案获得的富血小板纤维蛋白会影响与牙周炎相关的多物种龈下生物膜的形成。

Platelet-rich fibrin obtained from different protocols affects the formation of the multispecies subgingival biofilm associated with periodontitis.

作者信息

Hideaki Uyeda Fabio, Quilles Vargas Gustavo, Matias Malavazi Larissa, Tiemi Macedo Tatiane, Paim de Abreu Paulo Gomes Aline, Rocha Bueno Manuela, Henrique Moreira Paulo Tolentino Pedro, Aguiar da Silva Lucas Daylor, Cristina Figueiredo Luciene, Awad Shibli Jamil, Bueno-Silva Bruno

机构信息

Dental Research Division, Guarulhos University, Guarulhos, Brazil.

Department of Biosciences; Piracicaba School of Dentistry, University of Campinas, Piracicaba, Brazil.

出版信息

J Oral Microbiol. 2025 Jan 1;17(1):2445598. doi: 10.1080/20002297.2024.2445598. eCollection 2025.

DOI:10.1080/20002297.2024.2445598
PMID:39801747
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11722119/
Abstract

BACKGROUND

The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.

MATERIALS AND METHODS

PRF membranes and liquid-PRF were prepared using two protocols: centrifuge 1 uses fixed acceleration while centrifuge 2, progressive acceleration. PRF samples were introduced into device concurrently with 33-species bacterial inoculum. After seven days, biofilm metabolic activity (MA) and microbial profile were evaluated through colorimetric reaction and DNA-DNA hybridization, respectively.

RESULTS

Among PRF membranes, the ones from centrifuge 1 led to better reduction in MA, total biofilm, and F. periodonticum, P. gingivalis and T. forsythia counts when compared to untreated/centrifuge 2 treated biofilms. However, centrifuge 2 liquid-PRF reduced MA, total biofilm and F. periodonticum counts when compared to untreated/centrifuge 1 treated-biofilms.

CONCLUSION

PRF membrane and exhibited comparable antibiofilm activity. However, PRF distinct forms, obtained by same centrifugation protocol, may present different antimicrobial properties.

摘要

背景

本文旨在评估通过两种不同方案/离心机分别制备的富血小板纤维蛋白（PRF）膜不同部分以及液体PRF对多物种龈下生物膜的影响。

材料与方法

使用两种方案制备PRF膜和液体PRF：离心机1使用固定加速度，而离心机2使用渐进加速度。将PRF样本与33种细菌接种物同时引入装置中。七天后，分别通过比色反应和DNA-DNA杂交评估生物膜代谢活性（MA）和微生物谱。

结果

在PRF膜中，与未处理/离心机2处理的生物膜相比，离心机1制备的PRF膜能更好地降低MA、总生物膜以及牙周杆菌、牙龈卟啉单胞菌和福赛坦氏菌的数量。然而，与未处理/离心机1处理的生物膜相比，离心机2的液体PRF降低了MA、总生物膜和牙周杆菌的数量。

结论

PRF膜表现出相当的抗生物膜活性。然而，通过相同离心方案获得的PRF不同形式可能具有不同的抗菌特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/936c95581b12/ZJOM_A_2445598_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/36c1e06fb9c2/ZJOM_A_2445598_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/0bb0b7cc11bc/ZJOM_A_2445598_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/936c95581b12/ZJOM_A_2445598_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/36c1e06fb9c2/ZJOM_A_2445598_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/0bb0b7cc11bc/ZJOM_A_2445598_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a043/11722119/936c95581b12/ZJOM_A_2445598_F0003_OC.jpg

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