Lam Dinh Thi, Tanaka Katsunori, Takashima Akihiko, Shibutani Ayako, Ishikawa Ryuji
Faculty of Agriculture and Life Science, Hirosaki University Hirosaki Aomori Japan.
Historiographical Institute The University of Tokyo Bunkyo-ku Tokyo Japan.
Ecol Evol. 2025 Jan 9;15(1):e70828. doi: 10.1002/ece3.70828. eCollection 2025 Jan.
Paper mulberry is a fiber resource for paper making. Washi, a traditional paper in Japan, has been produced from × , a hybrid between and . Elite strains have been vegetatively propagated and distributed within Japan. In this study, these three strains' complete chloroplast (cp) genomes were reconstructed as pseudomolecules consisting of 160,861, 160,868, and 160,878 bp, respectively. They were compared with other database strains to detect insertion/deletion (INDEL) polymorphisms. In total, 25 INDELs were identified in these cp genomes. ISSR-based MIG-seq polymorphisms were applied to determine whether varieties or regional groups can be discriminated. Although various types of cultivars exhibiting different appearances are hard to discriminate, principal component analysis (PCA) distinguished two major groups. Maternal lineages reflecting the diverse origins of Japanese paper mulberry were determined using hypervariable maternal markers. An NGS-oriented nuclear marker system revealed the heterogeneous nature of domesticated paper mulberry, reflecting the complex origins of Japanese × .
构树是一种造纸纤维资源。日本的传统纸张和纸，是由构树与其他品种杂交培育出的杂交种×生产而来。优良品系已通过无性繁殖在日本国内推广。在本研究中，这三个品系的完整叶绿体（cp）基因组被重建为分别由160,861、160,868和160,878 bp组成的假分子。将它们与其他数据库品系进行比较，以检测插入/缺失（INDEL）多态性。在这些cp基因组中总共鉴定出25个INDEL。基于ISSR的MIG-seq多态性被用于确定是否可以区分品种或区域群体。尽管各种外观不同的栽培品种难以区分，但主成分分析（PCA）区分出了两个主要群体。利用高变母系标记确定了反映日本构树多样起源的母系谱系。一个面向NGS的核标记系统揭示了驯化构树的异质性，反映了日本杂交种×的复杂起源。
