Zhao Wei, Sun Xiaoxuan, Wu Shaoping, Wu Shuofan, Hu Chunhua, Huo Heqiang, Deng Guiming, Sheng Ou, Bi Fangcheng, He Weidi, Dou Tongxin, Dong Tao, Li Chunyu, Liu Siwen, Gao Huijun, Li Chunlong, Yi Ganjun, Yang Qiaosong
Institute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Science and Technology Research On Fruit Tree, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 Guangdong China.
Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510640 Guangdong China.
Mol Breed. 2025 Jan 9;45(1):12. doi: 10.1007/s11032-024-01523-3. eCollection 2025 Jan.
Previous studies illustrated that two banana GA20 oxidase2 (MaGA20ox2) genes, and , are implicated in controlling banana growth and development; however, the biological function of each gene remains unknown. Ma04g15900 protein (termed MaGA20ox2f in this article) is the closest homolog to the Rice SD1 (encoded by 'green revolution gene', ) in the banana genome. The expression of is confined to leaves, peduncles, fruit peels, and pulp. Knockout of by CRISPR/Cas9 led to late flowering and low-yielding phenotypes. The flowering time of #1 and #2 lines was delayed approximately by 61 and 58 days, respectively, while fruit yield decreased by 81.13% and 76.23% compared to wild type under normal conditions. The endogenous levels of downstream products of GA20 oxidase, GA15 and GA20, were significantly reduced in mutant shoots and fruits, but bioactive GA1 was only significantly reduced in the mutant fruits. Quantitative proteomics analysis identified 118 up-regulated proteins and 309 down-regulated proteins in both #1 and #2 lines, compared to wild type, with the down-regulated proteins primarily associated with photosynthesis, porphyrin and chlorophyll metabolism. The decreased chlorophyll contents in #1 and #2 lines corroborated the findings of the proteomics data. We propose that photosynthesis inhibition caused by lower chlorophyll contents in mutant leaves and GA1 deficiency in mutant fruits may be the two critical reasons contributing to the late flowering and low-yielding phenotypes of mutants.
The online version contains supplementary material available at 10.1007/s11032-024-01523-3.
先前的研究表明，两个香蕉GA20氧化酶2（MaGA20ox2）基因，即 和 ，参与调控香蕉的生长发育；然而，每个基因的生物学功能仍不清楚。Ma04g15900蛋白（本文中称为MaGA20ox2f）是香蕉基因组中与水稻SD1（由“绿色革命基因” 编码）最相似的同源物。 的表达局限于叶片、果梗、果皮和果肉。通过CRISPR/Cas9敲除 导致开花延迟和低产表型。#1和#2株系的开花时间分别延迟了约61天和58天，而在正常条件下，与野生型相比，果实产量分别下降了81.13%和76.23%。GA20氧化酶下游产物GA15和GA20的内源水平在 突变体的茎和果实中显著降低，但生物活性GA1仅在突变体果实中显著降低。定量蛋白质组学分析确定，与野生型相比，#1和#2株系中分别有118种上调蛋白和309种下调蛋白，下调蛋白主要与光合作用、卟啉和叶绿素代谢相关。#1和#2株系中叶绿素含量的降低证实了蛋白质组学数据的结果。我们认为， 突变体叶片中叶绿素含量降低导致的光合作用抑制和 突变体果实中GA1缺乏可能是导致 突变体开花延迟和低产表型的两个关键原因。
在线版本包含可在10.1007/s11032-024-01523-3获取的补充材料。
