Smith M R, Lequerica J L, Hart M R
J Bacteriol. 1985 Apr;162(1):67-71. doi: 10.1128/jb.162.1.67-71.1985.
NaCN was tested for its inhibitory effects on growth of and metabolism by Methanosarcina barkeri 227. NaCN (10 microM) inhibited catabolism of acetate methyl groups to CH4 and CO2 but did not inhibit methanogenesis from methanol, CO2, methylamine, or trimethylamine. NaCN also inhibited the assimilation of methanol or CO2 (as the sole carbon source) into cell carbon and stimulated the assimilation of acetate. These results suggest that inhibition by NaCN was a result of its action as an inhibitor of in vivo CO dehydrogenase. The results also implicate CO dehydrogenase in the oxidation of acetate but not methanol methyl groups to CO2.
对氰化钠(NaCN)抑制巴氏甲烷八叠球菌227生长及代谢的作用进行了测试。氰化钠(10微摩尔)抑制了乙酸甲酯基团分解为甲烷和二氧化碳的分解代谢,但不抑制甲醇、二氧化碳、甲胺或三甲胺生成甲烷的过程。氰化钠还抑制了甲醇或二氧化碳(作为唯一碳源)同化为细胞碳,并刺激了乙酸的同化作用。这些结果表明,氰化钠的抑制作用是其作为体内一氧化碳脱氢酶抑制剂发挥作用的结果。结果还表明一氧化碳脱氢酶参与乙酸氧化为二氧化碳的过程,但不参与甲醇甲基基团氧化为二氧化碳的过程。