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低温电镜筛选数据集分析的开源图形用户界面：低温电镜起重机

CryoCrane: an open-source GUI for analyzing cryo-EM screening data sets.

作者信息

Ruickoldt Jakob, Wendler Petra

机构信息

Institute for Biochemistry and Biology, University of Potsdam, Am Neuen Palais 10, 14469 Potsdam, Germany.

出版信息

Acta Crystallogr F Struct Biol Commun. 2025 Feb 1;81(Pt 2):62-65. doi: 10.1107/S2053230X25000081. Epub 2025 Jan 13.

DOI:10.1107/S2053230X25000081
PMID:39804567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11783181/
Abstract

Screening of cryo-EM samples is essential for the generation of high-resolution cryo-EM structures. Often, it is cumbersome to correlate the appearance of specific grid squares and micrograph quality. Here, CryoCrane (Correlate atlas and exposures), a visualization tool for cryo-EM screening data, is presented. It aims to provide an intuitive way to visualize micrographs and to speed up data analysis.

摘要

冷冻电镜样品的筛选对于生成高分辨率冷冻电镜结构至关重要。通常，将特定网格区域的外观与显微照片质量相关联很麻烦。在此，介绍了CryoCrane（关联图谱与曝光），一种用于冷冻电镜筛选数据的可视化工具。它旨在提供一种直观的方式来可视化显微照片并加快数据分析速度。

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本文引用的文献

1
Leginon: New features and applications.
Protein Sci. 2021 Jan;30(1):136-150. doi: 10.1002/pro.3967. Epub 2020 Nov 3.
2
Array programming with NumPy.
Nature. 2020 Sep;585(7825):357-362. doi: 10.1038/s41586-020-2649-2. Epub 2020 Sep 16.
3
Recent developments in the CCP-EM software suite.
Acta Crystallogr D Struct Biol. 2017 Jun 1;73(Pt 6):469-477. doi: 10.1107/S2059798317007859. Epub 2017 May 31.
4
Specimen Preparation for High-Resolution Cryo-EM.
Methods Enzymol. 2016;579:51-86. doi: 10.1016/bs.mie.2016.04.011. Epub 2016 Jun 16.
5
An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology.
Methods. 2016 May 1;100:3-15. doi: 10.1016/j.ymeth.2016.02.017. Epub 2016 Feb 28.
6
A primer to single-particle cryo-electron microscopy.
Cell. 2015 Apr 23;161(3):438-449. doi: 10.1016/j.cell.2015.03.050.
7
Plunge freezing for electron cryomicroscopy.
Methods Enzymol. 2010;481:63-82. doi: 10.1016/S0076-6879(10)81003-1.

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