Mahmoudi Ayda, Pour Vajiheh Zarrin, Salehzadeh Ali
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.
Sci Rep. 2025 Jan 13;15(1):1797. doi: 10.1038/s41598-025-85312-8.
The current chemotherapy treatments for liver cancer have shown limited effectiveness. Therefore, there is an urgent need to develop new drugs to combat this disease more effectively. This study reports synthesis of cobalt oxide nanoparticles coated with glucose, and conjugated with Ellagic acid. Physicochemical characterization of CoO@Glu-Ellagic acid nanoparticles was done using FT-IR, XRD, SEM, TEM, TGA, EDS-mapping, DLS, and zeta potential analyses, and the investigation of their anticancer potential on liver cancer cell lines involved the use of MTT, flow cytometry, and cell cycle analysis. The synthesized nanoparticles were somewhat spherical, arranged in a relatively cluster-shaped form, and were 33-46 nm in diameter. The zeta potential and particle hydrodynamic size were - 5.43 and 169 nm, respectively and had no elemental impurity. Also, the synthesized particles had proper thermal stability at temperatures below 100 °C. Treating cancer cells with the nanoparticles considerably increased ROS levels by 2.6 folds. Compared to normal human cells, CoO@Glu-Ellagic acid nanoparticles showed significantly higher toxicity for liver cancer and the 50% inhibitory concentration was 94 and 187 µg/mL for the cancer and normal cells, respectively. CoO@Glu-Ellagic acid increased cell apoptosis, from 0.87 to 9.24%, and the cells were mainly arrested at the G0/G1 and G2/M phases. Overall, the present work indicated that CoO@Glu-Ellagic acid has antiproliferative effects on liver cancer cells through an increased oxidative stress level, inhibition of cell cycle, and apoptosis induction.
目前用于肝癌的化疗治疗效果有限。因此，迫切需要开发新的药物来更有效地对抗这种疾病。本研究报道了包覆葡萄糖并与鞣花酸共轭的氧化钴纳米颗粒的合成。使用傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、热重分析（TGA）、能谱映射（EDS-mapping）、动态光散射（DLS）和zeta电位分析对CoO@Glu-鞣花酸纳米颗粒进行了物理化学表征，并且对其在肝癌细胞系上的抗癌潜力的研究涉及使用MTT、流式细胞术和细胞周期分析。合成的纳米颗粒呈 somewhat 球形，以相对簇状的形式排列，直径为33 - 46纳米。zeta电位和颗粒流体动力学尺寸分别为 - 5.43和169纳米，且无元素杂质。此外，合成的颗粒在低于100℃的温度下具有适当的热稳定性。用纳米颗粒处理癌细胞可使活性氧水平显著增加2.6倍。与正常人细胞相比，CoO@Glu-鞣花酸纳米颗粒对肝癌显示出明显更高的毒性，癌症细胞和正常细胞的50%抑制浓度分别为94和187μg/mL。CoO@Glu-鞣花酸使细胞凋亡从0.87%增加到9.24%，并且细胞主要停滞在G0/G1和G2/M期。总体而言，目前的工作表明CoO@Glu-鞣花酸通过增加氧化应激水平、抑制细胞周期和诱导凋亡对肝癌细胞具有抗增殖作用。
