通过多谱学、对接、细胞和抗菌方法探索钴氧化物纳米粒子与白蛋白、白血病癌细胞和病原菌的相互作用。
Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacteria by Multispectroscopic, Docking, Cellular and Antibacterial Approaches.
机构信息
Department of Biology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University (IAU), Lahijan, Guilan, Iran.
Department of Cellular and Molecular Biology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
出版信息
Int J Nanomedicine. 2020 Jun 25;15:4607-4623. doi: 10.2147/IJN.S257711. eCollection 2020.
AIM
The interaction of NPs with biological systems may reveal useful details about their pharmacodynamic, anticancer and antibacterial effects.
METHODS
Herein, the interaction of as-synthesized CoO NPs with HSA was explored by different kinds of fluorescence and CD spectroscopic methods, as well as molecular docking studies. Also, the anticancer effect of CoO NPs against leukemia K562 cells was investigated by MTT, LDH, caspase, real-time PCR, ROS, cell cycle, and apoptosis assays. Afterwards, the antibacterial effects of CoO NPs against three pathogenic bacteria were disclosed by antibacterial assays.
RESULTS
Different characterization methods such as TEM, DLS, zeta potential and XRD studies proved that fabricated CoO NPs by sol-gel method have a diameter of around 50 nm, hydrodynamic radius of 177 nm with a charge distribution of -33.04 mV and a well-defined crystalline phase. Intrinsic, extrinsic, and synchronous fluorescence as well as CD studies, respectively, showed that the HSA undergoes some fluorescence quenching, minor conformational changes, microenvironmental changes as well as no structural changes in the secondary structure, after interaction with CoO NPs. Molecular docking results also verified that the spherical clusters with a dimension of 1.5 nm exhibit the most binding energy with HSA molecules. Anticancer assays demonstrated that CoO NPs can selectively lead to the reduction of K562 cell viability through the cell membrane damage, activation of caspase-9, -8 and -3, elevation of Bax/Bcl-2 mRNA ratio, ROS production, cell cycle arrest, and apoptosis. Finally, antibacterial assays disclosed that CoO NPs can stimulate a promising antibacterial effect against pathogenic bacteria.
CONCLUSION
In general, these observations can provide useful information for the early stages of nanomaterial applications in therapeutic platforms.
目的
纳米粒子与生物系统的相互作用可能揭示其药效动力学、抗癌和抗菌作用的有用细节。
方法
本文通过各种荧光和 CD 光谱方法以及分子对接研究，探讨了合成的 CoO 纳米粒子与 HSA 的相互作用。此外，还通过 MTT、LDH、caspase、实时 PCR、ROS、细胞周期和凋亡测定研究了 CoO 纳米粒子对白血病 K562 细胞的抗癌作用。随后，通过抗菌试验揭示了 CoO 纳米粒子对三种病原菌的抗菌作用。
结果
不同的表征方法，如 TEM、DLS、zeta 电位和 XRD 研究表明，溶胶-凝胶法制备的 CoO 纳米粒子的直径约为 50nm，水动力半径为 177nm，带负电荷分布为-33.04mV，具有明确的结晶相。本征、外推和同步荧光以及 CD 研究分别表明，HSA 与 CoO 纳米粒子相互作用后，其荧光发生部分猝灭，构象发生轻微变化，微环境发生变化，但二级结构无结构变化。分子对接结果也验证了，尺寸为 1.5nm 的球形簇与 HSA 分子具有最高的结合能。抗癌试验表明，CoO 纳米粒子可以通过细胞膜损伤、激活 caspase-9、-8 和 -3、增加 Bax/Bcl-2mRNA 比值、产生 ROS、细胞周期停滞和凋亡，选择性地降低 K562 细胞活力。最后，抗菌试验揭示了 CoO 纳米粒子可以刺激对病原菌的有希望的抗菌作用。
结论
总的来说，这些观察结果可为纳米材料在治疗平台中的应用的早期阶段提供有用的信息。
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