Tao Yunming, Liu Jie, Qiu Wenxiao, Li Yuanyuan
Department of Thoracic Surgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17 Yongwaizheng Street, Donghu District, Nanchang, 330006, Jiangxi, China.
Surgery Department, Weifang Yidu Central Hospital, No.4138 Linglongshan South Road, Qingzhou, Weifang, 262500, Shandong, China.
Discov Oncol. 2025 Jan 13;16(1):40. doi: 10.1007/s12672-025-01739-5.
It is known that genomic instability contributes to cancer development. Mitotically associated long non-coding RNA (MANCR) has been reported to promote genomic stability, suggesting its involvement in cancers. Therefore, this study was conducted to investigate the role of MANCR in non-small cell lung cancer (NSCLC).
After NSCLC (n = 60) and control (healthy subjects, n = 60) plasma samples, as well as NSCLC and paired non-tumor tissues from patients were collected, the levels of MANCR expression in plasma and tissues was detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Then the correlations of MANCR expression with clinical stages were confirmed. The diagnostic values of MANCR in both plasma and tissue samples for stage I/II NSCLC were analyzed using Receiver Operating Characteristic (ROC) curves. All NSCLC patients were monitored for 5 years to investigate the role of MANCR in the prediction of patients' survival.
MANCR expression was downregulated in both NSCLC plasma and tissue of NSCLC patients compared to controls (P < 0.05). Decreased MANCR expression levels from stage I to IV were observed. However, MANCR expression in non-tumor tissue was not significantly different between different stages (P > 0.05). Additionally, stage I/II NSCLC patients were separated from controls using MANCR in plasma and tumor tissues as biomarkers. Lower MANCR levels in plasma and tumor were closely correlated with patients' higher mortality rate.
MANCR is down-expressed in NSCLC patients and may serve as a diagnostic and prognostic biomarker for NSCLC.
已知基因组不稳定会促进癌症发展。有报道称有丝分裂相关长链非编码RNA（MANCR）可促进基因组稳定性，提示其与癌症有关。因此，本研究旨在探讨MANCR在非小细胞肺癌（NSCLC）中的作用。
收集NSCLC患者（n = 60）和对照（健康受试者，n = 60）的血浆样本，以及患者的NSCLC组织和配对的非肿瘤组织，采用定量实时逆转录聚合酶链反应（qRT-PCR）检测血浆和组织中MANCR的表达水平。然后确定MANCR表达与临床分期的相关性。使用受试者工作特征（ROC）曲线分析血浆和组织样本中MANCR对I/II期NSCLC的诊断价值。对所有NSCLC患者进行5年随访，以研究MANCR在预测患者生存中的作用。
与对照组相比，NSCLC患者的NSCLC血浆和组织中MANCR表达均下调（P < 0.05）。观察到从I期到IV期MANCR表达水平降低。然而，不同分期的非肿瘤组织中MANCR表达无显著差异（P > 0.05）。此外，以血浆和肿瘤组织中的MANCR作为生物标志物，可将I/II期NSCLC患者与对照组区分开来。血浆和肿瘤中较低的MANCR水平与患者较高的死亡率密切相关。
MANCR在NSCLC患者中表达下调，可能作为NSCLC的诊断和预后生物标志物。
