Department of Integrated Medicine, Eastern Medical District of Chinese PLA General Hospital, Beijing, 100094, China.
Department of Health Services, The Seventh Medical Center of PLA General Hospital, Beijing, 100700, China.
BMC Pulm Med. 2021 Aug 21;21(1):272. doi: 10.1186/s12890-021-01635-y.
LncRNA MANCR (mitosis-related lncRNA, LINC00704) is deemed as a pivotal regulator in various cancers, yet the biological function it performs in lung adenocarcinoma (LUAD) was rarely reported. We made an in-depth study to clarify its effect during the progression of this cancer.
Expression data and clinical information were first accessed from TCGA LUAD dataset ( https://portal.gdc.cancer.gov/repository ). Differentially expressed lncRNAs were identified. R package "survival" determined the survival significance of the lncRNA MANCR. GSEA software was applied to conduct single sample enrichment analysis. qRT-PCR was used to examine MANCR expression. The expression levels of related proteins were tested using Western blot assay. The impact of MANCR on cancer cell biological behaviors was investigated via cell function experiments.
MANCR was significantly upregulated in LUAD cells. It also resulted in a poor prognosis. When MANCR expression was down-regulated, the expression of proteins related to invasion and migration, cell cycle and proliferation was decreased, while the expression of proteins associated with apoptosis was elevated. Furthermore, in vitro experiments revealed that silencing MANCR inhibited cancer cell functions, blocked cell cycle progression while promoting cell apoptosis.
LncRNA MANCR can lead to enhanced proliferative, invasive and migratory abilities of cancer cells while reducing cell apoptosis. Hence, MANCR might be a novel biomarker of LUAD.
LncRNA MANCR（有丝分裂相关 lncRNA，LINC00704）被认为是多种癌症中的关键调节因子，但它在肺腺癌（LUAD）中的生物学功能鲜有报道。我们进行了深入研究，以阐明其在这种癌症进展过程中的作用。
首先从 TCGA LUAD 数据集（https://portal.gdc.cancer.gov/repository）获取表达数据和临床信息。鉴定差异表达的 lncRNA。R 包“survival”确定 lncRNA MANCR 的生存意义。GSEA 软件用于进行单样本富集分析。qRT-PCR 用于检测 MANCR 表达。使用 Western blot 检测相关蛋白的表达水平。通过细胞功能实验研究 MANCR 对癌细胞生物学行为的影响。
MANCR 在 LUAD 细胞中显著上调。它还导致预后不良。下调 MANCR 表达时，与侵袭和迁移、细胞周期和增殖相关的蛋白表达降低，而与细胞凋亡相关的蛋白表达升高。此外，体外实验表明，沉默 MANCR 抑制了癌细胞功能，阻止了细胞周期进程，同时促进了细胞凋亡。
LncRNA MANCR 可以增强癌细胞的增殖、侵袭和迁移能力，同时减少细胞凋亡。因此，MANCR 可能是 LUAD 的一种新型生物标志物。
