Nie Qigui, Fang Xianfu, Huang Jiale, Xu Tingting, Li Yangfeng, Zhang Gong, Li Yizhou
Chongqing Fuling Hospital, Chongqing University, Chongqing, 40800, China.
Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.
Small Methods. 2025 Jan 13:e2401631. doi: 10.1002/smtd.202401631.
Deoxyribonucleic acid (DNA), a fundamental biomacromolecule in living organisms, serves as the carrier of genetic information. Beyond its role in encoding biological functions, DNA's inherent ability to hybridize through base pairing has opened new avenues for its application in biological sciences. This review introduces DNA nanotechnology and DNA-encoded library (DEL), and highlights their shared design principles related to DNA assembly. First, a foundational overview of structural DNA nanotechnology, including its design strategies and historical development is provided. Subsequently, various approaches are examined to dynamic DNA nanotechnology, from strand displacement reactions to DNA-templated polymer synthesis. Second, how the principle of DNA assembly has facilitated the development of diverse formats of self-assembly-based DEL synthesis, DNA-template reactions (DTS), and DNA template-mediated proximity induction effects are examined. These advancements are all underpinned by the unique property of DNA assembly. Finally, this review summarizes the common principles shared by DNA nanotechnology and DEL in terms of methodology and design. Additionally, the potential synergies are explored between these two technologies, envisioning future applications where they can be combined to create more versatile and exquisite functionalities.
脱氧核糖核酸（DNA）是生物体内一种基本的生物大分子，作为遗传信息的载体。除了在编码生物学功能方面的作用外，DNA通过碱基配对进行杂交的固有能力为其在生物科学中的应用开辟了新途径。本综述介绍了DNA纳米技术和DNA编码文库（DEL），并强调了它们与DNA组装相关的共同设计原则。首先，提供了结构DNA纳米技术的基础概述，包括其设计策略和历史发展。随后，研究了从链置换反应到DNA模板聚合物合成等动态DNA纳米技术的各种方法。其次，研究了DNA组装原理如何促进基于自组装的DEL合成、DNA模板反应（DTS）和DNA模板介导的邻近诱导效应等多种形式的发展。这些进展都基于DNA组装的独特性质。最后，本综述总结了DNA纳米技术和DEL在方法和设计方面的共同原则。此外，还探索了这两种技术之间的潜在协同作用，设想了未来可以将它们结合起来创造更通用、更精细功能的应用。
