Zohdi Rozaini M, Adli Muhammad A, Mukhtar Shahida M, Awang Junaidi Awang H, Bakar Md Zuki A
Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA, Selangor Branch, Puncak Alam, Selangor, Malaysia.
Department of Pharmacology and Life Sciences, Faculty of Pharmacy, Universiti Teknologi MARA, Selangor Branch, Puncak Alam, Selangor, Malaysia.
J Taibah Univ Med Sci. 2024 Dec 19;19(6):1181-1192. doi: 10.1016/j.jtumed.2024.12.008. eCollection 2024 Dec.
Concerns over the increasing number of obese individuals and the associated health risks have prompted therapeutic option explorations. Similarly, this study aimed to establish fruit extract (SCFE) anti-adipogenic attributes in 3T3-L1 cells.
The polyphenolic compounds in SCFE were identified with Reverse phase-high performance liquid chromatography (RP-HPLC). Meanwhile, murine 3T3-L1 preadipocytes, measuring leptin levels, reactive oxygen species (ROS), and lipid and triglyceride (TG) contents were utilized during anti-adipogenic activity assessments. Concurrently, the effects of SCFE on adipogenic transcription factors were established with quantitative real-time-polymerase chain reaction (qRT-PCR).
The RP-HPLC results indicated three polyphenolic compounds in SCFE, including one flavonoid (naringin) and two phenolic acids (syringic and p-coumaric). Although SCFE treatments (250-1000 μg/mL) did not result in cell toxicity, they significantly reduced dose-dependent lipid accumulation, ROS production, and TG and leptin levels relative to control-differentiated adipocytes. Moreover, SCFE suppressed sterol regulatory element binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR-γ), and CCAAT/enhancer-binding protein-alpha (C/EBP-α) gene expressions during preadipocyte differentiation into adipocytes.
The findings revealed the anti-adipogenic properties of SCFE, indicating its potential as a natural obesity management remedy. Nevertheless, more studies are necessary to elucidate the reactions resulting in SCFE anti-adipogenic effects and the active constituents responsible for the property.
对肥胖个体数量不断增加及其相关健康风险的担忧促使人们探索治疗方案。同样，本研究旨在确定水果提取物（SCFE）在3T3-L1细胞中的抗脂肪生成特性。
采用反相高效液相色谱（RP-HPLC）鉴定SCFE中的多酚化合物。同时，在抗脂肪生成活性评估过程中，利用小鼠3T3-L1前脂肪细胞检测瘦素水平、活性氧（ROS）以及脂质和甘油三酯（TG）含量。同时，通过定量实时聚合酶链反应（qRT-PCR）确定SCFE对脂肪生成转录因子的影响。
RP-HPLC结果表明SCFE中含有三种多酚化合物，包括一种黄酮类化合物（柚皮苷）和两种酚酸（丁香酸和对香豆酸）。尽管SCFE处理（250-1000μg/mL）未导致细胞毒性，但相对于对照分化的脂肪细胞，它们显著降低了剂量依赖性脂质积累、ROS产生以及TG和瘦素水平。此外，SCFE在脂肪前体细胞分化为脂肪细胞的过程中抑制了固醇调节元件结合蛋白-1（SREBP-1）、过氧化物酶体增殖物激活受体-γ（PPAR-γ）和CCAAT/增强子结合蛋白-α（C/EBP-α）基因的表达。
研究结果揭示了SCFE的抗脂肪生成特性，表明其作为天然肥胖管理药物的潜力。然而，需要更多的研究来阐明导致SCFE抗脂肪生成作用的反应以及具有该特性的活性成分。
