Saghghaei Farid, Rassouli Ali, Sadeghi-Hashjin Goudarz, Sasani Farhang, Koohi Mohammad Kazem
Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Vet Res Forum. 2024;15(11):613-620. doi: 10.30466/vrf.2024.2024154.4196. Epub 2024 Nov 15.
Pulmonary fibrosis (PF) is a chronic interstitial lung disease with a progressive damage to the air sacs and deposition of collagen fibers in the lung tissue. The study aimed to explore the effects of oil (NSO) or thymoquinone (TQ), alone or in combination with dexamethasone (DEX), on the development of bleomycin (BLM)-induced PF. Forty-two male rats were divided into seven groups: Control (CTRL); BLM, received a single dose of BLM on day 0, intratracheally; all remaining groups received BLM, as well. DEX, received DEX daily, intraperitoneally, 1 day before BLM and continued for 14 days; NSO and TQ groups, received daily NSO and TQ, respectively, 7 days before BLM and continued for 35 days; DEX + TQ, received both DEX and TQ; DEX + NSO, received both DEX and NSO. At the end, lung tissues were used for histopathological and biochemical analyses. BLM significantly increased the severity of fibrosis and inflammation compared to the CTRL. Bleomycin also significantly increased the amount of hydroxyproline, however, decreased most antioxidant enzymes in the lung tissue compared to the other groups. Group TQ + DEX significantly reduced the severity of BLM-induced PF as well as alterations in biochemical parameters, lung weight and O2 saturation. oil slightly reduced BLM-induced PF, however, it caused non-significant hyperemia in lung tissue. Thymoquinone potentiated the effects of DEX on most biochemical and pathological alterations of BLM-induced lung injury much better than NSO. More studies are needed to support the use of NSO and TQ as potential protective agents against PF.
肺纤维化（PF）是一种慢性间质性肺病，会对肺泡造成进行性损害，并在肺组织中沉积胶原纤维。该研究旨在探讨坚果籽油（NSO）或百里醌（TQ）单独或与地塞米松（DEX）联合使用对博来霉素（BLM）诱导的PF发展的影响。42只雄性大鼠被分为七组：对照组（CTRL）；BLM组，在第0天经气管内给予单剂量BLM；其余所有组也接受BLM。DEX组，在BLM给药前1天每天腹腔注射DEX，并持续14天；NSO组和TQ组，在BLM给药前7天分别每天给予NSO和TQ，并持续35天；DEX + TQ组，同时接受DEX和TQ；DEX + NSO组，同时接受DEX和NSO。最后，将肺组织用于组织病理学和生化分析。与CTRL组相比，BLM显著增加了纤维化和炎症的严重程度。博来霉素还显著增加了羟脯氨酸的含量，然而，与其他组相比，它降低了肺组织中大多数抗氧化酶的含量。TQ + DEX组显著降低了BLM诱导的PF的严重程度以及生化参数、肺重量和氧饱和度的改变。NSO略微减轻了BLM诱导的PF，然而，它导致肺组织出现不显著的充血。百里醌比NSO更好地增强了DEX对BLM诱导的肺损伤的大多数生化和病理改变的作用。需要更多的研究来支持将NSO和TQ用作预防PF的潜在保护剂。
