Watanabe Rika, Yoshida Takuma, Nakaminami Hidemasa
Department of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Access Microbiol. 2025 Jan 13;7(1). doi: 10.1099/acmi.0.000812.v4. eCollection 2025.
Antiseptics have been used for infection control against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Ethanol (EtOH) was found to be effective against SARS-CoV-2, while chlorhexidine gluconate (CHG) was less effective. Therefore, virucidal activity may differ between different classes of antiseptic agents. In this study, the efficacy of antiseptics against SARS-CoV-2 was evaluated, and effective agents for infection control were identified. The following antiseptics were used in this study: 1.5% olanexidine gluconate (OLG); 80% EtOH; 1% sodium hypochlorite (NaClO); 0.2% benzalkonium chloride (BKC); 1% povidone-iodine (PVP-I); 0.5%, 1% and 1.5% CHG; and 0.5% alkyldiaminoethylglycine hydrochloride (AEG). The virucidal activity was evaluated at 0, 0.5, 1, 2 and 3 min according to EN14476. After 0.5 min of exposure, 1.5% OLG, 80% EtOH, 1% NaClO, 0.2% BKC and 1% PVP-I inactivated SARS-CoV-2 below the detection limit. The virus survived in the presence of 0.5% CHG, 1% CHG or 0.5% AEG for 3 min. The virucidal activity of 1.5% CHG was insufficient after 0.5 min of exposure. The results showed that virucidal activity against SARS-CoV-2 differs depending on the class of antiseptic agents used under clean conditions. Despite belonging to the same class of biguanide antiseptics, OLG was more effective against SARS-CoV-2 than CHG.
已使用防腐剂来控制严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染。发现乙醇（EtOH）对SARS-CoV-2有效，而葡萄糖酸洗必泰（CHG）效果较差。因此，不同类别的防腐剂的杀病毒活性可能有所不同。在本研究中，评估了防腐剂对SARS-CoV-2的功效，并确定了有效的感染控制剂。本研究使用了以下防腐剂：1.5%葡萄糖酸奥替尼啶（OLG）；80%乙醇；1%次氯酸钠（NaClO）；0.2%苯扎氯铵（BKC）；1%聚维酮碘（PVP-I）；0.5%、1%和1.5%的CHG；以及0.5%盐酸烷基二氨基乙基甘氨酸（AEG）。根据EN14476在0、0.5、1、2和3分钟时评估杀病毒活性。暴露0.5分钟后，1.5%OLG、80%乙醇、1%NaClO、0.2%BKC和1%PVP-I将SARS-CoV-2灭活至检测限以下。病毒在0.5%CHG、1%CHG或0.5%AEG存在下存活3分钟。暴露0.5分钟后，1.5%CHG的杀病毒活性不足。结果表明，在清洁条件下，针对SARS-CoV-2的杀病毒活性因所用防腐剂的类别而异。尽管OLG和CHG属于同一类双胍类防腐剂，但OLG对SARS-CoV-2的效果比CHG更好。
