Li Jia-Qi, Wang Ai-Jun, Song Pei, Feng Jiu-Ju, Zhou Qi, Cheang Tuck Yun
Department of General Surgery, Hui Ya Hospital of The First Affiliated Hospital, Sun Yat-Sen University, Huizhou, 516081, Guangdong, China.
College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China.
Mikrochim Acta. 2025 Jan 15;192(2):82. doi: 10.1007/s00604-024-06892-2.
Self-supported ultrathin PtRuMoCoNi high-entropy alloy nanowires (HEANWs) were synthesized by a one-pot co-reduction method, whose peroxidase (POD)-like activity and catalytic mechanism were elaborated in detail. As expected, the PtRuMoCoNi HEANWs showed excellent POD-like activity. It can quickly catalyze the oxidization of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue TMB through decomposition of HO to superoxide radicals. Notably, isoniazid and hydrazine effectively scavenge the as-produced superoxide radicals and reduce the blue TMB, showing high reduction ability and antioxidant property. Thus, the PtRuMoCoNi HEANW-derived colorimetric method was developed for determination of isoniazid and hydrazine, which exhibited the linear ranges of 1.5 to 50 μM and 25 to 200 μM coupled with the lower detection limits of 2.3 and 12.6 μM for isoniazid and hydrazine, respectively. The excellent analytical performance mainly results from the synergistic catalytic effect of the multiple metals and distinctive ultra-thin nanowires. This work provides a simple and rapid colorimetric method for the determination of isoniazid and hydrazine in actual samples.
通过一锅共还原法合成了自支撑超薄PtRuMoCoNi高熵合金纳米线（HEANWs），并详细阐述了其类过氧化物酶（POD）活性和催化机制。正如预期的那样，PtRuMoCoNi HEANWs表现出优异的类POD活性。它可以通过将HO分解为超氧自由基，快速催化无色的3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色TMB。值得注意的是，异烟肼和肼能有效清除产生的超氧自由基并还原蓝色TMB，表现出高还原能力和抗氧化性能。因此，开发了基于PtRuMoCoNi HEANW的比色法用于测定异烟肼和肼，其线性范围分别为1.5至50μM和25至200μM，异烟肼和肼的检测限分别为2.3和12.6μM。优异的分析性能主要源于多种金属的协同催化作用和独特的超薄纳米线。这项工作为实际样品中异烟肼和肼的测定提供了一种简单快速的比色法。
