Demiray Aydın, Karagur Ege Rıza, Tel-Cayan Gulsen, Tokgun Onur, Akca Hakan, Duru Mehmet Emin
Department of Medical Genetic, School of Medicine, Pamukkale University, Denizli, Turkey.
Department of Chemistry and Chemical Processing Technologies, Muğla Vocational School, Muğla Sıtkı Koçman University, Muğla, Menteşe/Muğla, Turkey.
Anticancer Agents Med Chem. 2025;25(10):711-718. doi: 10.2174/0118715206349668241118104255.
Lung cancer is the primary cause of cancer-related deaths globally. Protein kinase B (AKT) protein is associated with many pathways in non-small cell lung cancer (NSCLC), such as proliferation, migration, invasion, and apoptosis. Mushrooms have a long history of being used in traditional medicine to treat various diseases. Scientists have been exploring the potential of mushrooms for their antioxidant and anticancer properties. In our study, the anti-oxidant, invasion, and apoptosis effects of mushroom extracts were investigated in NSCLC.
Non-Small Cell Lung Cancer cell lines H1299, PC-3, and PC-14 were used in our study. After obtaining the extracts of and , IC value was calculated as 25.04-11.73-16.54 ng/ul for and 2.97-1.53-1.01 μg/ul for , respectively, in H1299, PC3 and PC14 cell lines. Afterward, proliferative and invasion effects, as well as apoptosis and anti-oxidant effects, were investigated using the IC dose. Western blotting was performed to investigate the pathways of these effects.
According to the results of our study, and extracts have anti-proliferative and anti-invasive effects on non-small lung cancer cell lines and induced apoptosis, which has been found to increase the anti-oxidant effect. It was found that this effect was due to cross-talk between antioxidant activity and the AKT-Rb pathway.
We anticipate that and extracts will be effective in cancer treatment by suppressing lung cancer cells p-Akt and Rb.
肺癌是全球癌症相关死亡的主要原因。蛋白激酶B（AKT）蛋白与非小细胞肺癌（NSCLC）的许多途径相关，如增殖、迁移、侵袭和凋亡。蘑菇在传统医学中用于治疗各种疾病已有很长历史。科学家们一直在探索蘑菇的抗氧化和抗癌特性。在我们的研究中，研究了蘑菇提取物在非小细胞肺癌中的抗氧化、侵袭和凋亡作用。
我们的研究使用了非小细胞肺癌细胞系H1299、PC-3和PC-14。获得[提取物名称1]和[提取物名称2]的提取物后，在H1299、PC3和PC14细胞系中，[提取物名称1]的IC值分别计算为25.04 - 11.73 - 16.54 ng/ul，[提取物名称2]的IC值分别计算为2.97 - 1.53 - 1.01 μg/ul。随后，使用IC剂量研究增殖和侵袭作用以及凋亡和抗氧化作用。进行蛋白质印迹法以研究这些作用的途径。
根据我们的研究结果，[提取物名称1]和[提取物名称2]提取物对非小肺癌细胞系具有抗增殖和抗侵袭作用，并诱导凋亡，已发现其可增加抗氧化作用。发现这种作用是由于抗氧化活性与AKT - Rb途径之间的相互作用。
我们预计[提取物名称1]和[提取物名称2]提取物将通过抑制肺癌细胞的p - Akt和Rb在癌症治疗中有效。
