Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland.
Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey.
Molecules. 2022 Jan 3;27(1):275. doi: 10.3390/molecules27010275.
Fungal mycelium cultures are an alternative to natural sources in order to obtain valuable research materials. They also enable constant control and adaptation of the process, thereby leading to increased biomass growth and accumulation of bioactive metabolites. The present study aims to assess the biosynthetic potential of mycelial cultures of six species: , , , , , and . The presence of phenolic acids, amino acids, indole compounds, sterols, and kojic acid in biomass extracts was determined by HPLC. The antioxidant and cytotoxic activities of the extracts and their effects on the inhibition of selected enzymes (tyrosinase and acetylcholinesterase) were also evaluated. The total content of phenolic acids in the extracts ranged from 5.8 () to 114.07 mg/100 g dry weight (d.w.) (). The total content of indole compounds in the extracts ranged from 3.03 () to 11.56 mg/100 g d.w. () and that of ergosterol ranged from 28.15 () to 74.78 mg/100 g d.w. (). Kojic acid was found in the extracts of and . The tested extracts showed significant antioxidant activity. The results suggest that the analyzed mycelial cultures are promising candidates for the development of new dietary supplements or pharmaceutical preparations.
真菌菌丝体培养物是天然来源的替代品，以获取有价值的研究材料。它们还能够对过程进行持续控制和适应，从而导致生物量生长增加和生物活性代谢物的积累。本研究旨在评估六种 物种的菌丝体培养物的生物合成潜力：, ,,,, 和. 通过 HPLC 测定生物量提取物中酚酸、氨基酸、吲哚化合物、固醇和曲酸的存在。还评估了提取物的抗氧化和细胞毒性活性及其对抑制选定酶（酪氨酸酶和乙酰胆碱酯酶）的影响。提取物中酚酸的总含量范围为 5.8 () 至 114.07 mg/100 g 干重（d.w.）()。提取物中吲哚化合物的总含量范围为 3.03 () 至 11.56 mg/100 g d.w. ()，麦角固醇的含量范围为 28.15 () 至 74.78 mg/100 g d.w. ()。曲酸存在于 和 的提取物中。测试的提取物表现出显著的抗氧化活性。结果表明，分析的菌丝体培养物是开发新的膳食补充剂或药物制剂的有前途的候选物。
