从 Rhizopogon luteolus 和 Ganoderma adspersum 蘑菇中分离、结构表征和生物活性的半乳甘露聚糖。
Isolation, structural characterization, and biological activities of galactomannans from Rhizopogon luteolus and Ganoderma adspersum mushrooms.
机构信息
Department of Chemistry and Chemical Processing Technologies, Muğla Vocational School, Muğla Sıtkı Koçman University, 48000 Muğla, Turkey.
Department of Chemistry, Islamia College University Peshawar, Peshawar 25120, Pakistan; Department of Chemistry, Faculty of Sciences, Muğla Sıtkı Koçman University, 48000 Muğla, Turkey.
出版信息
Int J Biol Macromol. 2020 Dec 15;165(Pt B):2395-2403. doi: 10.1016/j.ijbiomac.2020.10.040. Epub 2020 Oct 13.
Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans I and II mainly include D-mannose and D-galactose in the molar percentages of 0.81:1.0 and 1:1.4, respectively. The GPC calculation demonstrated that the molecular weights are about 5240 and 5090 Da, respectively. Their structures comprise of β-(1,4)-mannose (Man) backbone units with α-(1,6)-galactose (Gal) single unit as a side group. The anticholinesterase activity of galactomannans was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), spectrophotometrically. Antioxidant activity was also measured by six assays (ABTS, DPPH, O, β-carotene-linoleic acid, metal chelating, and CUPRAC assays). Galactomannan II indicated close inhibitory activity to galantamine against AChE (61.04 ± 0.45%) and BChE (59.70 ± 1.15%) at 50 μg/mL concentration. Nevertheless, both galactomannans showed low antioxidant activity in all tests. This study reveals that mainly, Galactomannan II could be used as a new natural promising anticholinesterase agent.
多糖是蘑菇生物活性所必需的化合物。两种新的半乳甘露聚糖（Galactomannan I 和 II）从 R. luteolus 和 G. adspersum 中分离得到。使用 FT-IR、1D 和 2D-NMR 技术对其结构进行了表征。两种分离得到的半乳甘露聚糖 I 和 II 主要包含摩尔百分比为 0.81:1.0 和 1:1.4 的 D-甘露糖和 D-半乳糖。GPC 计算表明分子量分别约为 5240 和 5090 Da。它们的结构由β-(1,4)-甘露糖（Man）主链单元和α-(1,6)-半乳糖（Gal）单糖作为侧基组成。通过分光光度法测试了半乳甘露聚糖对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制活性。还通过六种测定法（ABTS、DPPH、O、β-胡萝卜素-亚油酸、金属螯合和CUPRAC 测定法）测量了抗氧化活性。半乳甘露聚糖 II 在 50 µg/mL 浓度下对 AChE（61.04 ± 0.45%）和 BChE（59.70 ± 1.15%）具有与加兰他敏接近的抑制活性。然而，两种半乳甘露聚糖在所有测试中均表现出较低的抗氧化活性。本研究表明，半乳甘露聚糖 II 主要可以用作新的有前途的天然乙酰胆碱酯酶抑制剂。
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