Hodinka L, Merétey K, Jancsó A, Falus A, Korda J, Bozsóky S
Arch Immunol Ther Exp (Warsz). 1979;27(5):641-6.
Sera of patients with symptoms of connective tissue diseases were investigated for the presence of antinuclear antibodies by indirect immunofluorescence on rat liver substrate. In positive cases antibodies to native DNA were determined by radioimmunoassay and by counterimmunoelectrophoresis. Twenty seven selected sera were also tested by indirect immunofluorescence on Crithidia luciliae substrate to test the sensitivity and specificity of this method. Immunofluorescent antinuclear antibodies could be found in all groups of connective tissue diseases while anti-native DNA antibody was demonstrated in higher amount only in the cases of systemic lupus erythematosus and therefore determination of anti-native DNA antibody may be helpful in the diagnosis of oligosymptomatic SLE. Counterimmunoelectrophoresis and immunofluorescence on Crithidia smears may serve as screening methods for anti-native DNA while radioimmunoassay provides a quantitative determination, although there are minor differences in the sensitivity and the specificity of these three methods.
通过对大鼠肝脏底物进行间接免疫荧光法，研究了有结缔组织疾病症状患者血清中抗核抗体的存在情况。在阳性病例中，通过放射免疫测定法和对流免疫电泳法测定抗天然DNA抗体。还通过对克氏锥虫底物进行间接免疫荧光法对27份选定的血清进行检测，以测试该方法的灵敏度和特异性。在所有结缔组织疾病组中均可发现免疫荧光抗核抗体，而仅在系统性红斑狼疮病例中抗天然DNA抗体的含量较高，因此抗天然DNA抗体的测定可能有助于寡症状性系统性红斑狼疮的诊断。对流免疫电泳法和克氏涂片免疫荧光法可作为抗天然DNA的筛查方法，而放射免疫测定法可进行定量测定，尽管这三种方法在灵敏度和特异性上存在细微差异。
