Sontheimer R D, Gilliam J N
J Lab Clin Med. 1978 Apr;91(4):550-8.
Circulating antibodies to dsDNA are found predominately in SLE and are seen most often in those patients with active systemic disease, particularly severe lupus glomerulonephritis. An IIF technique for measuring these antibodies has recently been described. This uncomplicated assay employs the kinetoplast of the nonpathogenic hemoflagellate Crithidia lucilliae as a dsDNA substrate. We herein report our experience with this assay, emphasizing the methodology and interpretation of results. Although slightly less sensitive than a radioimmunoassay, we find that this IF technique is a specific and reliable qualitative method for detecting anti-dsDNA. An estimate of the amount of DNA antibody present can be obtained by serum titration. The test was positive in only two patient groups tested, SLE (48%) and MCTD (20%).
双链DNA循环抗体主要在系统性红斑狼疮（SLE）患者中发现，且最常见于有活动性全身性疾病的患者，尤其是重症狼疮性肾小球肾炎患者。最近描述了一种用于检测这些抗体的间接免疫荧光（IIF）技术。这种简单的检测方法采用非致病性血鞭毛虫路氏锥虫的动基体作为双链DNA底物。我们在此报告我们使用该检测方法的经验，重点介绍方法和结果解读。虽然该方法的敏感性略低于放射免疫测定法，但我们发现这种免疫荧光技术是检测抗双链DNA的一种特异性且可靠的定性方法。通过血清滴定可估计存在的DNA抗体量。在仅测试的两个患者组中该检测呈阳性反应，即SLE（48%）和混合性结缔组织病（MCTD，20%）。
