Wei Wei, Wang Long-Fei, Tao Jian-Jun, Zhang Wan-Ke, Chen Shou-Yi, Song Qingxin, Zhang Jin-Song
Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, China.
J Integr Plant Biol. 2025 Mar;67(3):649-668. doi: 10.1111/jipb.13834. Epub 2025 Jan 17.
Plant oils play a crucial role in human nutrition, industrial applications and biofuel production. While the enzymes involved in fatty acid (FA) biosynthesis are well-studied, the regulatory networks governing these processes remain largely unexplored. This review explores the intricate regulatory networks modulating seed oil biosynthesis, focusing on key pathways and factors. Seed oil content is determined by the efficiency of de novo FA synthesis as well as influenced by sugar transport, lipid metabolism, FA synthesis inhibitors and fine-tuning mechanisms. At the center of this regulatory network is WRINKLED1 (WRI1), which plays a conserved role in promoting seed oil content across various plant species. WRI1 interacts with multiple proteins, and its expression level is regulated by upstream regulators, including members of the LAFL network. Beyond the LAFL network, we also discuss a potential nuclear factor-Y (NF-Y) regulatory network in soybean with an emphasis on NF-YA and NF-YB and their associated proteins. This NF-Y network represents a promising avenue for future efforts aimed at enhancing oil accumulation and improving stress tolerance in soybean. Additionally, the application of omics-based approaches is of great significance. Advances in omics technologies have greatly facilitated the identification of gene resources, opening new opportunities for genetic improvement. Importantly, several transcription factors involved in oil biosynthesis also participate in stress responses, highlighting a potential link between the two processes. This comprehensive review elucidates the complex mechanisms underlying the regulation of oil biosynthesis, offering insights into potential biotechnological strategies for improving oil production and stress tolerance in oil crops.
植物油在人类营养、工业应用和生物燃料生产中发挥着关键作用。虽然参与脂肪酸（FA）生物合成的酶已得到充分研究，但控制这些过程的调控网络在很大程度上仍未被探索。本综述探讨了调节种子油生物合成的复杂调控网络，重点关注关键途径和因素。种子油含量由从头合成FA的效率决定，并受糖运输、脂质代谢、FA合成抑制剂和微调机制的影响。该调控网络的核心是皱叶1（WRI1），它在促进不同植物物种的种子油含量方面发挥着保守作用。WRI1与多种蛋白质相互作用，其表达水平受上游调节因子调控，包括LAFL网络的成员。除了LAFL网络，我们还讨论了大豆中一个潜在的核因子-Y（NF-Y）调控网络，重点是NF-YA和NF-YB及其相关蛋白质。这个NF-Y网络是未来旨在提高大豆油积累和改善胁迫耐受性的努力的一个有前景的途径。此外，基于组学方法的应用具有重要意义。组学技术的进步极大地促进了基因资源的鉴定，为遗传改良开辟了新机会。重要的是，一些参与油生物合成的转录因子也参与胁迫反应，突出了这两个过程之间的潜在联系。这篇全面的综述阐明了油生物合成调控的复杂机制，为提高油作物产量和胁迫耐受性的潜在生物技术策略提供了见解。
