Department of Pediatrics, Suqian Hospital Affiliated to Xuzhou Medical University, Suqian, Jiangsu, 223800, People's Republic of China.
BMC Infect Dis. 2024 Nov 21;24(1):1331. doi: 10.1186/s12879-024-10233-2.
The association between alterations in the oral microbiome and hand, foot, and mouth disease (HFMD) has been observed in previous studies. Our study, therefore, aimed to identify the structural changes in the oral microbiota and biomarkers in children with HFMD caused by enterovirus A 71 (EV-A71).
Children diagnosed with EV-A71 HFMD and healthy children recruited from April 2021 to September 2023 were included in the present study, and were categorized into EV-A71 and control groups, respectively. Oral swabs were collected and microbiota information was obtained using 16 S rRNA gene sequencing technology. Alpha-diversity and partial least squares discriminant analyses were conducted to compare microbial diversity, richness, and similarity between the two groups. Linear discriminant analysis effect size was employed to identify microbial taxa with significant differences, and determined the key genera among them.
The study included a total of 80 children, with 50 assigned to the EV-A71 group and 30 to the control group. No significant differences were found between the two groups in terms of age (2.2 ± 1.2 vs. 2.7 ± 1.2 years; age range: 1-5 years; P = 0.114) or sex (56% vs. 60% boys, P = 0.726). The oral microbiota structure in the EV-A71 group differed from that in the control group. The EV-A71 group showed significant reductions in both the Shannon index (P = 0.037) and the abundance-based coverage estimator (ACE) index (P < 0.001). The key genus changes were marked by a significant decrease in the abundance of Capnocytophaga (P = 0.002) and Leptotrichia (P = 0.033) in the EV-A71 group.
In children with EV-A71 HFMD, the oral microbiota showed changes in composition, with a significant reduction in diversity and richness. The changes in key genera were a marked decrease in the abundance of Capnocytophaga and Leptotrichia.
先前的研究已经观察到口腔微生物组的改变与手足口病（HFMD）之间存在关联。因此，我们的研究旨在确定由肠道病毒 A71（EV-A71）引起的手足口病患儿口腔微生物群和生物标志物的结构变化。
本研究纳入了 2021 年 4 月至 2023 年 9 月期间诊断为 EV-A71 手足口病的患儿和健康儿童，并将其分别归入 EV-A71 组和对照组。采集口腔拭子，采用 16S rRNA 基因测序技术获取微生物组信息。采用 alpha 多样性和偏最小二乘判别分析比较两组间微生物多样性、丰富度和相似性。采用线性判别分析效应量识别微生物分类群的显著差异，并确定其中的关键属。
本研究共纳入 80 名儿童，其中 50 名归入 EV-A71 组，30 名归入对照组。两组在年龄（2.2±1.2 岁 vs. 2.7±1.2 岁；年龄范围：1-5 岁；P=0.114）或性别（56% vs. 60%男孩，P=0.726）方面无显著差异。EV-A71 组的口腔微生物群结构与对照组不同。EV-A71 组的 Shannon 指数（P=0.037）和 ACE 指数（P<0.001）均显著降低。关键属的变化表现为 Capnocytophaga（P=0.002）和 Leptotrichia（P=0.033）丰度显著降低。
在 EV-A71 手足口病患儿中，口腔微生物群组成发生变化，多样性和丰富度显著降低。关键属的变化表现为 Capnocytophaga 和 Leptotrichia 的丰度显著降低。
