Zhao Yi, Zhao Min, Zhang Wen
Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Front Vet Sci. 2025 Jan 3;11:1457471. doi: 10.3389/fvets.2024.1457471. eCollection 2024.
To fully characterize papillomavirus diversity in giant pandas (), we identified a novel papillomavirus (named AmPV5, GenBank accession number MZ357114) in oral swabs from giant pandas with the help of viral metagenomics technology in this study. The complete circular genome of AmPV5 is 7,935 bp in length, with a GC content of 39.1%. It encodes five early genes (E1, E2, E4, E6, and E7), two late genes (L1 and L2), and features conserved zinc-binding domains (CXXC- (X)28/29-CXXC) in E6 and E7 genes. E7 protein has an LxCxE domain (pRB binding) in its N-terminal region. The nucleotide sequence of AmPV5 L1 gene shares < 70% identity with other related sequences available in the GenBank database. Phylogenetic analysis indicated that AmPV5 fell within the genus but formed a monophyletic branch away from other papillomaviruses found in , and . According to the International Committee on Taxonomy of Viruses (ICTV) classification guidelines, AmPV5 is classified as a new species within the genus. The discovery provides valuable insights into the viral diversity in giant pandas and highlights the need for continued surveillance of wildlife pathogens. Future studies should explore the potential role of AmPV5 in the health and disease ecology of this endangered species.
为全面描述大熊猫乳头瘤病毒的多样性，在本研究中，我们借助病毒宏基因组学技术，从大熊猫的口腔拭子中鉴定出一种新型乳头瘤病毒（命名为AmPV5，GenBank登录号MZ357114）。AmPV5的完整环状基因组长度为7935 bp，GC含量为39.1%。它编码五个早期基因（E1、E2、E4、E6和E7）、两个晚期基因（L1和L2），并且在E6和E7基因中具有保守的锌结合结构域（CXXC-(X)28/29-CXXC）。E7蛋白在其N端区域有一个LxCxE结构域（pRB结合结构域）。AmPV5 L1基因的核苷酸序列与GenBank数据库中其他相关序列的同一性小于70%。系统发育分析表明，AmPV5属于 属，但形成了一个单系分支，与在 、 和 中发现的其他乳头瘤病毒不同。根据国际病毒分类委员会（ICTV）的分类指南，AmPV5被归类为 属内的一个新物种。这一发现为大熊猫病毒多样性提供了有价值的见解，并突出了持续监测野生动物病原体的必要性。未来的研究应探索AmPV5在这种濒危物种的健康和疾病生态中的潜在作用。
