Hu Yu-Qi, Liu Kang, Lai Le-Qing, He Yi-Ru, Hong Li-Ping, Jiang Chu-Qian, Liu Si-Min, Cao Ming-Zhu
Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Department of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, China.
Front Med (Lausanne). 2025 Jan 6;11:1467530. doi: 10.3389/fmed.2024.1467530. eCollection 2024.
In clinical practice, an accurate and efficient detection approach for pulmonary tuberculosis (PTB) is highly needed. The fluorescence hybridization (FISH) assay for PTB might be a suitable alternative to current tests. However, a systematic assessment of the diagnostic performance of this new approach is not available. Our study aimed to determine the diagnostic accuracy of FISH for PTB.
We examined PubMed and three more databases including Embase, Cochrane Library, and Web of Science databases from their establishment to November 10, 2023, for published articles on the diagnostic performance of FISH on individuals with clinical suspicion of tuberculosis (TB). QUADAS-2 was used to evaluate the literature's quality. We used Meta-DiSc software to create forest plots.
The search yielded 7 studies, involving 1,224 sputum samples that could be included in our meta-analysis. The combined FISH sensitivity and specificity were 0.89 (95% CI 0.86-0.92) and 0.98 (95% CI 0.97-0.99), respectively. Furthermore, subgroup analysis was performed based on probes and PTB incidence.
FISH may be useful in the diagnosis of pulmonary tuberculosis. The sensitivity and specificity of FISH are high for most sputum specimens. Additionally, FISH has better diagnostic performance in countries with low PTB prevalence than in high PTB prevalence countries. We hope this study will find a new and effective tool for the early diagnosis of PTB.
在临床实践中，非常需要一种准确、高效的肺结核（PTB）检测方法。用于PTB的荧光杂交（FISH）检测可能是当前检测方法的合适替代方法。然而，目前尚无对这种新方法诊断性能的系统评估。我们的研究旨在确定FISH检测PTB的诊断准确性。
我们检索了PubMed以及另外三个数据库，包括Embase、Cochrane图书馆和Web of Science数据库，检索时间从其建立至2023年11月10日，以查找关于FISH对临床怀疑患有结核病（TB）个体诊断性能的已发表文章。使用QUADAS-2评估文献质量。我们使用Meta-DiSc软件制作森林图。
检索共得到7项研究，涉及1224份痰标本，可纳入我们的荟萃分析。FISH的合并敏感性和特异性分别为0.89（95%CI 0.86 - 0.92）和0.98（95%CI 0.97 - 0.99）。此外，还根据探针和PTB发病率进行了亚组分析。
FISH可能对肺结核的诊断有用。对于大多数痰标本，FISH的敏感性和特异性较高。此外，与PTB高流行率国家相比，FISH在PTB低流行率国家具有更好的诊断性能。我们希望这项研究能找到一种用于PTB早期诊断的新的有效工具。
