Beinart Freya R, Gillen Kathy
Kenyon College, Molecular Biology, Gambier, Ohio, USA.
Kenyon College, Biology, Gambier, Ohio, USA.
Dev Growth Differ. 2025 Feb;67(2):104-112. doi: 10.1111/dgd.12961. Epub 2025 Jan 21.
Animals vary in their ability to replace body parts lost to injury, a phenomenon known as restorative regeneration. Uncovering conserved signaling steps required for regeneration may aid regenerative medicine. Reactive oxygen species (ROS) are necessary for proper regeneration in species across a wide range of taxa, but it is unknown whether ROS are essential for annelid regeneration. As annelids are a widely used and excellent model for regeneration, we sought to determine whether ROS play a role in the regeneration of the highly regenerative annelid, Lumbriculus variegatus. Using a ROS-sensitive fluorescent probe we observed ROS accumulation at the wound site within 15 min after amputation; this ROS burst lessened by 6 h post-amputation. Chemical inhibition of this ROS burst delayed regeneration, an impairment that was partially rescued with exogenous ROS. Our results suggest that similar to other animals, annelid regeneration depends upon ROS signaling, implying a phylogenetically ancient requirement for ROS in regeneration.
动物在替换因受伤而失去的身体部位的能力上存在差异，这一现象被称为修复性再生。揭示再生所需的保守信号传导步骤可能有助于再生医学。活性氧（ROS）对于广泛分类群中的物种进行适当再生是必需的，但尚不清楚ROS对于环节动物的再生是否至关重要。由于环节动物是一种广泛使用且出色的再生模型，我们试图确定ROS是否在高度可再生的环节动物颤蚓的再生中发挥作用。使用对ROS敏感的荧光探针，我们观察到截肢后15分钟内伤口部位有ROS积累；这种ROS爆发在截肢后6小时减弱。对这种ROS爆发的化学抑制会延迟再生，而外源性ROS可部分挽救这种损伤。我们的结果表明，与其他动物类似，环节动物的再生依赖于ROS信号传导，这意味着在系统发育上，再生对ROS的需求由来已久。
