McFrederick Quinn S, Vuong Hoang Q, Rothman Jason A
Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA.
Graduate Program in Microbiology, University of California, Riverside, Riverside, CA 92521, USA.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1879-1884. doi: 10.1099/ijsem.0.002758. Epub 2018 Apr 12.
Gram-stain-positive, rod-shaped, non-spore forming bacteria have been isolated from flowers and the guts of adult wild bees in the families Megachilidae and Halictidae. Phylogenetic analysis of the 16S rRNA gene indicated that these bacteria belong to the genus Lactobacillus, and are most closely related to the honey-bee associated bacteria Lactobacillus kunkeei (97.0 % sequence similarity) and Lactobacillus apinorum (97.0 % sequence similarity). Phylogenetic analyses of 16S rRNA genes and six single-copy protein coding genes, in situ and in silico DNA-DNA hybridization, and fatty-acid profiling differentiates the newly isolated bacteria as three novel Lactobacillus species: Lactobacillus micheneri sp. nov. with the type strain Hlig3 (=DSM 104126,=NRRL B-65473), Lactobacillus timberlakei with the type strain HV_12 (=DSM 104128,=NRRL B-65472), and Lactobacillus quenuiae sp. nov. with the type strain HV_6 (=DSM 104127,=NRRL B-65474).
已从切叶蜂科和隧蜂科成年野生蜜蜂的花朵和肠道中分离出革兰氏染色阳性、杆状、不形成芽孢的细菌。对16S rRNA基因的系统发育分析表明，这些细菌属于乳杆菌属，与蜜蜂相关细菌昆氏乳杆菌（序列相似性为97.0%）和蜜蜂乳杆菌（序列相似性为97.0%）关系最为密切。对16S rRNA基因和六个单拷贝蛋白质编码基因的系统发育分析、原位和计算机DNA-DNA杂交以及脂肪酸谱分析将新分离的细菌鉴定为三个新的乳杆菌物种：模式菌株为Hlig3（=DSM 104126，=NRRL B-65473）的米氏乳杆菌、模式菌株为HV_12（=DSM 104128，=NRRL B-65472）的廷伯莱克乳杆菌以及模式菌株为HV_6（=DSM 104127，=NRRL B-65474）的凯纽乳杆菌。
