Imai Hiroyuki, Hyoto Muneyoshi, Fujino Kaoru, Matsuya Sumito, Nishida Akari, Aoyagi Ryoji, Tsutsumi Yunosuke, Kano Kiyoshi, Kusakabe Ken Takeshi
Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Laboratory of Veterinary Anatomy, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
J Vet Med Sci. 2025 Mar 1;87(3):277-279. doi: 10.1292/jvms.24-0516. Epub 2025 Jan 21.
The three-drug anesthetic mixture (medetomidine, midazolam and butorphanol), developed as an injectable anesthetic for laboratory animals, has been verified from various perspectives and applied to mice and other laboratory animals. However, the effects of its storage conditions and periods on its efficacy have not yet been studied. This study investigated the mixture's efficacy after storage under various conditions (room temperature, 4°C and -20°C) for 1 and 2 years. Mice in all groups were induced into a stable anesthetic state for at least 15 min after administration. The mice recovered from the anesthetized state 35 min after administration of the antagonist. These findings demonstrate the mixture's stability under different storage conditions and durations, potentially improving laboratory mouse welfare.
作为一种用于实验动物的注射用麻醉剂而研发的三药麻醉合剂（美托咪定、咪达唑仑和布托啡诺），已从多个角度得到验证，并应用于小鼠及其他实验动物。然而，其储存条件和储存时间对药效的影响尚未得到研究。本研究调查了该合剂在不同条件（室温、4℃和-20℃）下储存1年和2年后的药效。给药后，所有组的小鼠均被诱导进入稳定麻醉状态至少15分钟。给予拮抗剂后35分钟，小鼠从麻醉状态苏醒。这些研究结果证明了该合剂在不同储存条件和时长下的稳定性，可能会改善实验小鼠的健康状况。
