Ochi Takehiro, Nishiura Ippei, Tatsumi Mitsuyoshi, Hirano Yoshimi, Yahagi Kouichi, Sakurai Yasuhiro, Matsuyama-Fujiwara Kanako, Sudo Yuji, Nishina Noriko, Koyama Hironari
Laboratory Animal Science Div., Astellas Research Technologies Co., Ltd., 1-6 Kashima 2-chome, Yodogawa-ku, Osaka 532-8514, Japan.
J Vet Med Sci. 2014 Jun;76(6):917-21. doi: 10.1292/jvms.13-0589. Epub 2014 Mar 3.
The anesthetic effect of a combination of medetomidine, midazolam and butorphanol (Me-Mi-Bu) was evaluated in healthy cynomolgus monkeys. The Me-Mi-Bu combination was intramuscularly administered as follows: Dose 1, Me 0.015 mg/kg-Mi 0.1 mg/kg-Bu 0.15 mg/kg; Dose 2, Me 0.02 mg/kg-Mi 0.15 mg/kg-Bu 0.2 mg/kg; and Dose 3, Me 0.04 mg/kg-Mi 0.3 mg/kg-Bu 0.4 mg/kg. The combination rapidly induced immobilization, and lateral recumbency was reached within 15 min. The duration of anesthesia for each dose administered was follows: Dose 1, 47 ± 27 min; Dose 2, 113 ± 31 min; and Dose 3, 190 ± 24 min. The anesthetic effect of the combination was abolished by the α2-adrenoceptor antagonist atipamezole. No marked changes in the levels of hematologic or serum biochemical parameters were noted in cynomolgus monkeys administered the combination plus atipamezole. Taken together, these results suggest that the Me-Mi-Bu combination exhibits reversible anesthetic effect and may be useful for studies involving cynomolgus monkeys.
在健康食蟹猴中评估了美托咪定、咪达唑仑和布托啡诺（Me-Mi-Bu）联合使用的麻醉效果。Me-Mi-Bu联合用药通过肌肉注射给药，具体如下：剂量1，美托咪定0.015mg/kg-咪达唑仑0.1mg/kg-布托啡诺0.15mg/kg；剂量2，美托咪定0.02mg/kg-咪达唑仑0.15mg/kg-布托啡诺0.2mg/kg；剂量3，美托咪定0.04mg/kg-咪达唑仑0.3mg/kg-布托啡诺0.4mg/kg。联合用药迅速诱导动物出现制动，15分钟内达到侧卧姿势。各给药剂量的麻醉持续时间如下：剂量1，47±27分钟；剂量2，113±31分钟；剂量3，190±24分钟。α2-肾上腺素能受体拮抗剂阿替美唑可消除联合用药的麻醉效果。在给予联合用药加阿替美唑的食蟹猴中，未观察到血液学或血清生化参数水平有明显变化。综上所述，这些结果表明Me-Mi-Bu联合用药具有可逆性麻醉作用，可能对涉及食蟹猴的研究有用。
